biostudies-literatureYang XNational Natural Science Foundation of ChinaChina Postdoctoral Science Foundation24673-24678https://www.ebi.ac.uk/biostudies/studies/S-EPMC9082159biostudies-literatureUnknown8(43)Microbial lipids (MLs) are potential alternatives to vegetable oils and animal fats for production of biofuels and oleochemicals. It remains critical to improve ML production efficiency and costs for further commercial development. In the present study, we overexpressed a gene encoding phosphotransacetylase (Pta) in the oleaginous yeast <i>Rhodosporidium toruloides</i> for enhanced cell growth and lipid production. Compared with the parental strain <i>R. toruloides</i> NP11, the engineered strain NP-Pta-15 showed significant improvement in glucose consumption, cell growth and lipid accumulation when cultivated under nitrogen limited conditions in an Erlenmeyer flask as well as a stirred tank bioreactor. The introduction of Pta may establish an additional acetyl-CoA formation route by utiliRSC advancesExpression of phosphotransacetylase in <i>Rhodosporidium toruloides</i> leading to improved cell growth and lipid production.PMC90821592016M591469213256275176114501421602218Yang XZhao ZKSun WZhang SShen HJiao XfalseExpression of phosphotransacetylase in <i>Rhodosporidium toruloides</i> leading to improved cell growth and lipid production.Microbial lipids (MLs) are potential alternatives to vegetable oils and animal fats for production of biofuels and oleochemicals. It remains critical to improve ML production efficiency and costs for further commercial development. In the present study, we overexpressed a gene encoding phosphotransacetylase (Pta) in the oleaginous yeast <i>Rhodosporidium toruloides</i> for enhanced cell growth and lipid production. Compared with the parental strain <i>R. toruloides</i> NP11, the engineered strain NP-Pta-15 showed significant improvement in glucose consumption, cell growth and lipid accumulation when cultivated under nitrogen limited conditions in an Erlenmeyer flask as well as a stirred tank bioreactor. The introduction of Pta may establish an additional acetyl-CoA formation route by utili2018-01-01T00:00:00Z2018 Jul2025-04-22T07:50:19.246Z2025-04-05T22:17:48.16ZS-EPMC90821593553919810.1039/c8ra03028f