{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nanao Y"],"funding":["BLRD VA","NHLBI NIH HHS","NIGMS NIH HHS"],"pagination":["111613"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9082579"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["548"],"pubmed_abstract":["DNA methylation alteration is tissue-specific and play a pivotal role in regulating gene transcription during cell proliferation and survival. We aimed to detect genes regulated by DNA methylation, and then investigated whether the gene influenced cell proliferation or survival in adrenal cells. DNA methylation and qPCR analyses were performed in nonfunctioning adrenocortical adenoma (NFA, n = 12) and aldosterone-producing adenoma (APA, n = 35) samples. The VDR gene promoter was markedly hypomethylated in APA with ATP1A1 mutation, and the promoter methylation levels showed a significant inverse association with the transcripts in APA. ATP1A1 mutation led to VDR transcription in HAC15 cells, and VDR suppression abrogated ATP1A1 mutation-mediated cell proliferation in HAC15 cells. We demonst"],"journal":["Molecular and cellular endocrinology"],"pubmed_title":["Hypomethylation associated vitamin D receptor expression in ATP1A1 mutant aldosterone-producing adenoma."],"pmcid":["PMC9082579"],"funding_grant_id":["I01 BX004681","R01 HL144847","U54 GM115428"],"pubmed_authors":["Yoshii Y","Gomez-Sanchez CE","Otagaki Y","Nanao Y","Arihiro K","Okada A","Nagano G","Oki K","Kodama T","Baba R","Kobuke K","Itcho K","Yoneda M","Hattori N","Ohno H"],"additional_accession":[]},"is_claimable":false,"name":"Hypomethylation associated vitamin D receptor expression in ATP1A1 mutant aldosterone-producing adenoma.","description":"DNA methylation alteration is tissue-specific and play a pivotal role in regulating gene transcription during cell proliferation and survival. We aimed to detect genes regulated by DNA methylation, and then investigated whether the gene influenced cell proliferation or survival in adrenal cells. DNA methylation and qPCR analyses were performed in nonfunctioning adrenocortical adenoma (NFA, n = 12) and aldosterone-producing adenoma (APA, n = 35) samples. The VDR gene promoter was markedly hypomethylated in APA with ATP1A1 mutation, and the promoter methylation levels showed a significant inverse association with the transcripts in APA. ATP1A1 mutation led to VDR transcription in HAC15 cells, and VDR suppression abrogated ATP1A1 mutation-mediated cell proliferation in HAC15 cells. We demonst","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-20T00:12:51.888Z","creation":"2025-04-20T00:12:51.888Z"},"accession":"S-EPMC9082579","cross_references":{"pubmed":["35257799"],"doi":["10.1016/j.mce.2022.111613"]}}