{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma C"],"funding":["National Natural Science Foundation of China"],"pagination":["899578"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9085562"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Directed evolution is a widely-used engineering strategy for improving the stabilities or biochemical functions of proteins by repeated rounds of mutation and selection. A protein of interest is selected as the template and expressed on a molecular display platform such as a bacteriophage for engineering. Initially, the surface-displayed protein template needs to be checked against the desired target <i>via</i> ELISA to examine whether the functions of the displayed template remain intact. The ELISA signal is subject to the protein-target binding affinity. A low-affinity results in a weak ELISA signal which makes it difficult to determine whether the weak signal is because of low affinity or because of poor expression of the protein. Using a methyllysine-binding chromodomain protein Cbx1 t"],"journal":["Frontiers in microbiology"],"pubmed_title":["Development in Detection Methods for the Expression of Surface-Displayed Proteins."],"pmcid":["PMC9085562"],"funding_grant_id":["31770821","32071430"],"pubmed_authors":["Ma C","Li S","Jiang C","Zhao D","Li R","Li L"],"additional_accession":[]},"is_claimable":false,"name":"Development in Detection Methods for the Expression of Surface-Displayed Proteins.","description":"Directed evolution is a widely-used engineering strategy for improving the stabilities or biochemical functions of proteins by repeated rounds of mutation and selection. A protein of interest is selected as the template and expressed on a molecular display platform such as a bacteriophage for engineering. Initially, the surface-displayed protein template needs to be checked against the desired target <i>via</i> ELISA to examine whether the functions of the displayed template remain intact. The ELISA signal is subject to the protein-target binding affinity. A low-affinity results in a weak ELISA signal which makes it difficult to determine whether the weak signal is because of low affinity or because of poor expression of the protein. Using a methyllysine-binding chromodomain protein Cbx1 t","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-04T10:02:21.665Z","creation":"2025-04-04T10:02:21.665Z"},"accession":"S-EPMC9085562","cross_references":{"pubmed":["35558116"],"doi":["10.3389/fmicb.2022.899578"]}}