biostudies-literatureMa CNational Natural Science Foundation of China899578https://www.ebi.ac.uk/biostudies/studies/S-EPMC9085562biostudies-literatureUnknown13Directed evolution is a widely-used engineering strategy for improving the stabilities or biochemical functions of proteins by repeated rounds of mutation and selection. A protein of interest is selected as the template and expressed on a molecular display platform such as a bacteriophage for engineering. Initially, the surface-displayed protein template needs to be checked against the desired target <i>via</i> ELISA to examine whether the functions of the displayed template remain intact. The ELISA signal is subject to the protein-target binding affinity. A low-affinity results in a weak ELISA signal which makes it difficult to determine whether the weak signal is because of low affinity or because of poor expression of the protein. Using a methyllysine-binding chromodomain protein Cbx1 tFrontiers in microbiologyDevelopment in Detection Methods for the Expression of Surface-Displayed Proteins.PMC90855623177082132071430Ma CLi SJiang CZhao DLi RLi LfalseDevelopment in Detection Methods for the Expression of Surface-Displayed Proteins.Directed evolution is a widely-used engineering strategy for improving the stabilities or biochemical functions of proteins by repeated rounds of mutation and selection. A protein of interest is selected as the template and expressed on a molecular display platform such as a bacteriophage for engineering. Initially, the surface-displayed protein template needs to be checked against the desired target <i>via</i> ELISA to examine whether the functions of the displayed template remain intact. The ELISA signal is subject to the protein-target binding affinity. A low-affinity results in a weak ELISA signal which makes it difficult to determine whether the weak signal is because of low affinity or because of poor expression of the protein. Using a methyllysine-binding chromodomain protein Cbx1 t2022-01-01T00:00:00Z20222025-04-04T10:02:21.665Z2025-04-04T10:02:21.665ZS-EPMC90855623555811610.3389/fmicb.2022.899578