{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(14)"],"submitter":["Nag S"],"funding":["University Grants Commission","Council of Scientific and Industrial Research","Department of Science and Technology, Government of West Bengal"],"pubmed_abstract":["Gold nanoparticle formulated tannic acid (AuNP-TA) was synthesized, and its anticancer activity was compared to that of free tannic acid (TA). The half maximal inhibitory concentration (IC<sub>50</sub>) was reduced by half when cell lines were treated with AuNP-TA as compared to IC<sub>50</sub> values upon free TA treatment. Both showed better cytotoxic activity in HCT116 cell line as compared to MCF7 and HepG2. AuNP-TA induced death of HCT116 cells was associated with characteristic apoptotic changes. At the same treatment dose, AuNP-TA generated more ROS, caused a more extensive DNA damage and promoted higher expression of p53 and p21 than TA. Treatment with AuNP-TA regulated generation of p53 and ROS bi-directionally. Binding studies showed that TA lowered the expression of Akt, which inhibited the survival of colon cancer cells. Also, cell cycle arrest at the G2/M phase, enhanced expression of caspase-3/9, Bak, and Bax, loss of mitochondrial membrane potential, and enhanced level of cytosolic cytochrome <i>c</i> were observed in AuNP-TA treated HCT116 cells. Thus, AuNP-TA is more efficient than TA in inducing apoptotic cell death of HCT116 cells <i>via</i> the ROS/P53/Akt axis."],"journal":["RSC advances"],"pagination":["8025-8038"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9087445"],"repository":["biostudies-literature"],"pubmed_title":["Tannic acid-stabilized gold nano-particles are superior to native tannic acid in inducing ROS-dependent mitochondrial apoptosis in colorectal carcinoma cells <i>via</i> the p53/AKT axis."],"pmcid":["PMC9087445"],"pubmed_authors":["Saha KD","Nag S","Manna K"],"additional_accession":[]},"is_claimable":false,"name":"Tannic acid-stabilized gold nano-particles are superior to native tannic acid in inducing ROS-dependent mitochondrial apoptosis in colorectal carcinoma cells <i>via</i> the p53/AKT axis.","description":"Gold nanoparticle formulated tannic acid (AuNP-TA) was synthesized, and its anticancer activity was compared to that of free tannic acid (TA). The half maximal inhibitory concentration (IC<sub>50</sub>) was reduced by half when cell lines were treated with AuNP-TA as compared to IC<sub>50</sub> values upon free TA treatment. Both showed better cytotoxic activity in HCT116 cell line as compared to MCF7 and HepG2. AuNP-TA induced death of HCT116 cells was associated with characteristic apoptotic changes. At the same treatment dose, AuNP-TA generated more ROS, caused a more extensive DNA damage and promoted higher expression of p53 and p21 than TA. Treatment with AuNP-TA regulated generation of p53 and ROS bi-directionally. Binding studies showed that TA lowered the expression of Akt, which inhibited the survival of colon cancer cells. Also, cell cycle arrest at the G2/M phase, enhanced expression of caspase-3/9, Bak, and Bax, loss of mitochondrial membrane potential, and enhanced level of cytosolic cytochrome <i>c</i> were observed in AuNP-TA treated HCT116 cells. Thus, AuNP-TA is more efficient than TA in inducing apoptotic cell death of HCT116 cells <i>via</i> the ROS/P53/Akt axis.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Mar","modification":"2024-11-20T23:15:45.134Z","creation":"2024-11-20T23:15:45.134Z"},"accession":"S-EPMC9087445","cross_references":{"pubmed":["35547831"],"doi":["10.1039/c9ra00808j"]}}