{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gu Y"],"funding":["National Natural Science Foundation of China"],"pagination":["35855-35862"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9088196"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(62)"],"pubmed_abstract":["<i>Microthrix parvicella</i> (<i>M. parvicella</i>) is a filamentous bacterium that induces bulking in activated sludge. Here, we used the affinity of long-chain fatty acids (LCFA) for <i>M. parvicella</i> to create a novel fluorescent probe of carbazole modified by LCFA. The structure was characterized by <sup>1</sup>H NMR spectroscopy and mass spectrometry. The spectral properties, photostability, and hydrophobic properties of the probe were also characterized. Fluorescent-labeling results showed that it can label <i>M. parvicella in situ</i> and could be biodegraded <i>via</i> metabolism. The stable docking mode of carbazole probes with different fatty acid chains and lipases was also docked by the density functional tight-binding (DFTB) method."],"journal":["RSC advances"],"pubmed_title":["A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of <i>Microthrix parvicella</i>."],"pmcid":["PMC9088196"],"funding_grant_id":["11604238","51578360","21502137","51778398"],"pubmed_authors":["Ren X","Gu Y","Lin D","Wang C","Fei X","Yang Q","Tang Y"],"additional_accession":[]},"is_claimable":false,"name":"A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of <i>Microthrix parvicella</i>.","description":"<i>Microthrix parvicella</i> (<i>M. parvicella</i>) is a filamentous bacterium that induces bulking in activated sludge. Here, we used the affinity of long-chain fatty acids (LCFA) for <i>M. parvicella</i> to create a novel fluorescent probe of carbazole modified by LCFA. The structure was characterized by <sup>1</sup>H NMR spectroscopy and mass spectrometry. The spectral properties, photostability, and hydrophobic properties of the probe were also characterized. Fluorescent-labeling results showed that it can label <i>M. parvicella in situ</i> and could be biodegraded <i>via</i> metabolism. The stable docking mode of carbazole probes with different fatty acid chains and lipases was also docked by the density functional tight-binding (DFTB) method.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Oct","modification":"2025-04-04T10:01:41.488Z","creation":"2025-04-04T10:01:41.488Z"},"accession":"S-EPMC9088196","cross_references":{"pubmed":["35547900"],"doi":["10.1039/c8ra06986g"]}}