{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Rojas L"],"funding":["Vetenskapsrådet"],"pubmed_abstract":["<i>Giardia intestinalis</i> is a protozoan parasite causing diarrheal disease, giardiasis, after extracellular infection of humans and other mammals' intestinal epithelial cells (IECs) of the upper small intestine. The parasite has two main life cycle stages: replicative trophozoites and transmissive cysts. Differentiating parasites (encysting cells) and trophozoites have recently been shown to be present in the same regions of the upper small intestine, whereas most mature cysts are found further down in the intestinal system. To learn more about host-parasite interactions during <i>Giardia</i> infections, we used an <i>in vitro</i> model of the parasite's interaction with host IECs (differentiated Caco-2 cells) and <i>Giardia</i> WB trophozoites, early encysting cells (7 h), and cysts. D"],"journal":["Frontiers in cellular and infection microbiology"],"pagination":["862211"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9094438"],"repository":["biostudies-literature"],"pubmed_title":["Dual RNA Sequencing Reveals Key Events When Different <i>Giardia</i> Life Cycle Stages Interact With Human Intestinal Epithelial Cells <i>In Vitro</i>."],"pmcid":["PMC9094438"],"pubmed_authors":["Rojas L","Ma'ayeh S","Xu F","Gruttner J","Svard SG"],"additional_accession":[]},"is_claimable":false,"name":"Dual RNA Sequencing Reveals Key Events When Different <i>Giardia</i> Life Cycle Stages Interact With Human Intestinal Epithelial Cells <i>In Vitro</i>.","description":"<i>Giardia intestinalis</i> is a protozoan parasite causing diarrheal disease, giardiasis, after extracellular infection of humans and other mammals' intestinal epithelial cells (IECs) of the upper small intestine. The parasite has two main life cycle stages: replicative trophozoites and transmissive cysts. Differentiating parasites (encysting cells) and trophozoites have recently been shown to be present in the same regions of the upper small intestine, whereas most mature cysts are found further down in the intestinal system. To learn more about host-parasite interactions during <i>Giardia</i> infections, we used an <i>in vitro</i> model of the parasite's interaction with host IECs (differentiated Caco-2 cells) and <i>Giardia</i> WB trophozoites, early encysting cells (7 h), and cysts. D","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-05-30T20:54:36.976Z","creation":"2025-04-04T10:02:04.212Z"},"accession":"S-EPMC9094438","cross_references":{"pubmed":["35573800"],"doi":["10.3389/fcimb.2022.862211"]}}