biostudies-literatureCerezo-Cortes MIMINCIENCIAS, Colombia, partially support to Patricia del Portillo and Juan Manuel AnzolaUniversidad Nacional de Colombia, Facultad de Medicina, Proyecto Hermes. Partially support the Doctoral thesis of Maria Irene CerezoMinisterio de Ciencia, Tecnología e Inovación de Colombia, MINCIENCIASConsejo Nacional de Ciencia y Tecnología CONACyT, MéxicoMinisterio de Ciencia, Tecnología e Inovación de Colombia, MINCIENCIAS.5157https://www.ebi.ac.uk/biostudies/studies/S-EPMC9100210biostudies-literatureUnknown23(9)<i>Mycobacterium tuberculosis</i> (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated asInternational journal of molecular sciencesClose Related Drug-Resistance Beijing Isolates of <i>Mycobacterium tuberculosis</i> Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model.PMC9100210CV-2013-223279CT-80740-004-202053688 and 42665CT/731-2018Del Portillo PMurcia MIMata-Espinosa DABini EIZatarain-Barron ZLBarrios-Payan JHernandez-Pando RCerezo-Cortes MIOchoa-Leyva ARodriguez-Castillo JGAnzola JMCornejo-Granados FfalseClose Related Drug-Resistance Beijing Isolates of <i>Mycobacterium tuberculosis</i> Reveal a Different Transcriptomic Signature in a Murine Disease Progression Model.<i>Mycobacterium tuberculosis</i> (MTB) lineage 2/Beijing is associated with high virulence and drug resistance worldwide. In Colombia, the Beijing genotype has circulated since 1997, predominantly on the pacific coast, with the Beijing-Like SIT-190 being more prevalent. This genotype conforms to a drug-resistant cluster and shows a fatal outcome in patients. To better understand virulence determinants, we performed a transcriptomic analysis with a Beijing-Like SIT-190 isolate (BL-323), and Beijing-Classic SIT-1 isolate (BC-391) in progressive tuberculosis (TB) murine model. Bacterial RNA was extracted from mice lungs on days 3, 14, 28, and 60. On average, 0.6% of the total reads mapped against MTB genomes and of those, 90% against coding genes. The strains were independently associated as2022-01-01T00:00:00Z2022 May2026-05-08T03:10:43.509Z2024-10-16T11:36:34.923ZS-EPMC91002103556354510.3390/ijms23095157