biostudies-literatureElebring EErik and Lily Philipsons MemorialErik and Lily Philipson MemorialGothenburg Medical Association, Sahlgrenska University HospitalSahlgrenska UniversityhospitalGothenburg Medical Association1961https://www.ebi.ac.uk/biostudies/studies/S-EPMC9100393biostudies-literatureUnknown14(9)<h4>Background and aims</h4>Insights into the nature of gut adaptation after different diets enhance the understanding of how food modifications can be used to treat type 2 diabetes and obesity. The aim was to understand how diets, enriched in fat or carbohydrates, affect glucose absorption in the human healthy jejunum, and what mechanisms are involved.<h4>Methods</h4>Fifteen healthy subjects received, in randomised order and a crossover study design, two weeks of iso-caloric high-fat diet (HFD) and high-carbohydrate diet (HCD). Following each dietary period, jejunal mucosa samples were retrieved and assessed for protein expression using immunofluorescence and western blotting. Functional characterisation of epithelial glucose transport was assessed ex vivo using Ussing chambers. RegulatioNutrientsA Fatty Diet Induces a Jejunal Ketogenesis Which Inhibits Local SGLT1-Based Glucose Transport via an Acetylation Mechanism-Results from a Randomized Cross-Over Study between Iso-Caloric High-Fat versus High-Carbohydrate Diets in Healthy Volunteers.PMC9100393ALFGBG-673941, ALGBG721551MRC_MC_UU_12012/5ALFGBG721551MRC_ MC_UU_12012/5.ALFGBG-673941Docherty NGMarschall HUWallenius VFandriks LCasselbrant ARoux CWLElebring EfalseA Fatty Diet Induces a Jejunal Ketogenesis Which Inhibits Local SGLT1-Based Glucose Transport via an Acetylation Mechanism-Results from a Randomized Cross-Over Study between Iso-Caloric High-Fat versus High-Carbohydrate Diets in Healthy Volunteers.<h4>Background and aims</h4>Insights into the nature of gut adaptation after different diets enhance the understanding of how food modifications can be used to treat type 2 diabetes and obesity. The aim was to understand how diets, enriched in fat or carbohydrates, affect glucose absorption in the human healthy jejunum, and what mechanisms are involved.<h4>Methods</h4>Fifteen healthy subjects received, in randomised order and a crossover study design, two weeks of iso-caloric high-fat diet (HFD) and high-carbohydrate diet (HCD). Following each dietary period, jejunal mucosa samples were retrieved and assessed for protein expression using immunofluorescence and western blotting. Functional characterisation of epithelial glucose transport was assessed ex vivo using Ussing chambers. Regulatio2022-01-01T00:00:00Z2022 May2026-04-08T09:49:30.702Z2024-11-07T13:39:59.437ZS-EPMC91003933556592910.3390/nu14091961