biostudies-literatureLiu XHeilongjiang Tongsheng Food Technology Co., Ltd.,2664https://www.ebi.ac.uk/biostudies/studies/S-EPMC9102369biostudies-literatureUnknown27(9)This study aimed to elucidate the responses of a novel characterized Issatchenkia terricola WJL-G4 against citric acid stress by performing physiological analysis, morphology observation, and structural and membrane fatty acid composition analysis. The results showed that under citric acid stress, the cell vitality of I. terricola WJL-G4 was reduced. The cell morphology changed with the unclear, uncompleted and thinner cell wall, and degraded the cell structure. When the citric acid concentration was 20 g/L, I. terricola WJL-G4 could tolerate citric acid and maintain the cell structure by increasing the intracellular pH, superoxide dismutase activity, and contents of unsaturated fatty acids. As the citric acid concentration was ≥80 g/L, the stress has excMolecules (Basel, Switzerland)Responses of Issatchenkia terricola WJL-G4 upon Citric Acid Stress.PMC91023692019TSYF01Liu XBao YWang JTang YNing WLuo TfalseResponses of Issatchenkia terricola WJL-G4 upon Citric Acid Stress.This study aimed to elucidate the responses of a novel characterized Issatchenkia terricola WJL-G4 against citric acid stress by performing physiological analysis, morphology observation, and structural and membrane fatty acid composition analysis. The results showed that under citric acid stress, the cell vitality of I. terricola WJL-G4 was reduced. The cell morphology changed with the unclear, uncompleted and thinner cell wall, and degraded the cell structure. When the citric acid concentration was 20 g/L, I. terricola WJL-G4 could tolerate citric acid and maintain the cell structure by increasing the intracellular pH, superoxide dismutase activity, and contents of unsaturated fatty acids. As the citric acid concentration was ≥80 g/L, the stress has exc2022-01-01T00:00:00Z2022 Apr2025-04-04T20:35:28.186Z2025-04-04T20:35:28.186ZS-EPMC91023693556601510.3390/molecules27092664