{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kono J"],"funding":["NIDDK NIH HHS"],"pagination":["5037"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9102543"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(9)"],"pubmed_abstract":["Bladder inflammatory diseases cause various urinary symptoms, such as urinary frequency and painful urination, that impair quality of life. In this study, we used a mouse model of cyclophosphamide (CYP)-induced bladder inflammation and immortalized human urothelial (TRT-HU1) cells to explore the preventive potential of nobiletin (NOB), a polymethoxylated flavone enriched in citrus fruit peel, and investigate its mechanism of action in the bladder. Prophylaxis with PMF90 (60% NOB) attenuated the development of bladder inflammation and urinary symptoms in CYP-treated mice. PMF90 also reduced the upregulation of connexin 43 (Cx43), a major component of gap junction channels, in the bladder mucosa of CYP-treated mice. Stimulation of TRT-HU1 cells with the pro-inflammatory cytokine IL-1β increa"],"journal":["International journal of molecular sciences"],"pubmed_title":["Flavonoid Nobiletin Attenuates Cyclophosphamide-Induced Cystitis in Mice through Mechanisms That Involve Inhibition of IL-1β Induced Connexin 43 Upregulation and Gap Junction Communication in Urothelial Cells."],"pmcid":["PMC9102543"],"funding_grant_id":["R01 DK122153"],"pubmed_authors":["Ueda M","Negoro H","Kono J","Woo JT","Kobayashi T","Sengiku A","Suadicani SO","Ogawa O"],"additional_accession":[]},"is_claimable":false,"name":"Flavonoid Nobiletin Attenuates Cyclophosphamide-Induced Cystitis in Mice through Mechanisms That Involve Inhibition of IL-1β Induced Connexin 43 Upregulation and Gap Junction Communication in Urothelial Cells.","description":"Bladder inflammatory diseases cause various urinary symptoms, such as urinary frequency and painful urination, that impair quality of life. In this study, we used a mouse model of cyclophosphamide (CYP)-induced bladder inflammation and immortalized human urothelial (TRT-HU1) cells to explore the preventive potential of nobiletin (NOB), a polymethoxylated flavone enriched in citrus fruit peel, and investigate its mechanism of action in the bladder. Prophylaxis with PMF90 (60% NOB) attenuated the development of bladder inflammation and urinary symptoms in CYP-treated mice. PMF90 also reduced the upregulation of connexin 43 (Cx43), a major component of gap junction channels, in the bladder mucosa of CYP-treated mice. Stimulation of TRT-HU1 cells with the pro-inflammatory cytokine IL-1β increa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-18T15:03:20.975Z","creation":"2025-02-19T00:55:47.344Z"},"accession":"S-EPMC9102543","cross_references":{"pubmed":["35563427"],"doi":["10.3390/ijms23095037"]}}