biostudies-literatureSahu SUniversity Grants Commission2859https://www.ebi.ac.uk/biostudies/studies/S-EPMC9103770biostudies-literatureUnknown27(9)Optical chemosensors caused a revolution in the field of sensing due to their high specificity, sensitivity, and fast detection features. Imidazole derivatives have offered promising features in the literature as they bear suitable donor/acceptor groups for the selective analytes in the skeleton. In this work, an isoindole-imidazole containing a Schiff base chemosensor (1-{3-[(2-Diethylamino-ethylimino)-methyl]-2-hydroxy-5-methyl-phenyl}-2H-imidazo[5,1-a]isoindole-3,5-dione) was designed and synthesized. The complete sensing phenomena have been investigated by means of UV-Vis, fluorescence, lifetime measurement, FT-IR, NMR and ESI-MS spectroscopic techniques. The optical properties of the synthesized ligand were investigated in 3:7 HEPES buffer:DMSO medium and found to be highly selective and sensitive toward Zn²⁺ ion through a fluorescence turn-on response with detection limit of 0.073 μm. Furthermore, this response is effective in gel form also. The competition studies reveal that the response of the probe for Zn²⁺ ion is unaffected by other relevant metal ions. The stoichiometric binding study was performed utilizing Job's method which indicated a 1:1 sensor-Zn²⁺ ensemble. Computational calculations were performed to pinpoint the mechanism of sensing.Molecules (Basel, Switzerland)Turn on Fluorescence Sensing of Zn²⁺ Based on Fused Isoindole-Imidazole Scaffold.PMC9103770F1‒17.1/2017‒18/RGNF‒2017‒18‒SC‒WES‒37660/(SA‒III/Website)Cerezo JGoswami SSahu SBag RCeron-Carrasco JPSikdar YfalseTurn on Fluorescence Sensing of Zn²⁺ Based on Fused Isoindole-Imidazole Scaffold.Optical chemosensors caused a revolution in the field of sensing due to their high specificity, sensitivity, and fast detection features. Imidazole derivatives have offered promising features in the literature as they bear suitable donor/acceptor groups for the selective analytes in the skeleton. In this work, an isoindole-imidazole containing a Schiff base chemosensor (1-{3-[(2-Diethylamino-ethylimino)-methyl]-2-hydroxy-5-methyl-phenyl}-2H-imidazo[5,1-a]isoindole-3,5-dione) was designed and synthesized. The complete sensing phenomena have been investigated by means of UV-Vis, fluorescence, lifetime measurement, FT-IR, NMR and ESI-MS spectroscopic techniques. The optical properties of the synthesized ligand were investigated in 3:7 HEPES buffer:DMSO medium and found to be highly selective and sensitive toward Zn²⁺ ion through a fluorescence turn-on response with detection limit of 0.073 μm. Furthermore, this response is effective in gel form also. The competition studies reveal that the response of the probe for Zn²⁺ ion is unaffected by other relevant metal ions. The stoichiometric binding study was performed utilizing Job's method which indicated a 1:1 sensor-Zn²⁺ ensemble. Computational calculations were performed to pinpoint the mechanism of sensing.2022-01-01T00:00:00Z2022 Apr2025-04-18T17:07:22.833Z2025-02-19T00:56:20.027ZS-EPMC91037703556621110.3390/molecules27092859