biostudies-literatureUnknown27(9)Dadiotis EThe widespread use of phytocannabinoids or cannabis extracts as ingredients in numerous types of products, in combination with the legal restrictions on THC content, has created a need for the development of new, rapid, and universal analytical methods for their quantitation that ideally could be applied without separation and standards. Based on previously described qNMR studies, we developed an expanded ¹H qNMR method and a novel 2D-COSY qNMR method for the rapid quantitation of ten major phytocannabinoids in cannabis plant extracts and cannabis-based products. The ¹H qNMR method was successfully developed for the quantitation of cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabichromene (CBC), cannabichromenic acid (CBCA), cannabigerol (CBG), cMolecules (Basel, Switzerland)2965https://www.ebi.ac.uk/biostudies/studies/S-EPMC9103933biostudies-literatureDirect Quantitation of Phytocannabinoids by One-Dimensional ¹H qNMR and Two-Dimensional ¹H-¹H COSY qNMR in Complex Natural Mixtures.PMC9103933Magiatis PDadiotis EMelliou EMitsis VfalseDirect Quantitation of Phytocannabinoids by One-Dimensional ¹H qNMR and Two-Dimensional ¹H-¹H COSY qNMR in Complex Natural Mixtures.The widespread use of phytocannabinoids or cannabis extracts as ingredients in numerous types of products, in combination with the legal restrictions on THC content, has created a need for the development of new, rapid, and universal analytical methods for their quantitation that ideally could be applied without separation and standards. Based on previously described qNMR studies, we developed an expanded ¹H qNMR method and a novel 2D-COSY qNMR method for the rapid quantitation of ten major phytocannabinoids in cannabis plant extracts and cannabis-based products. The ¹H qNMR method was successfully developed for the quantitation of cannabidiol (CBD), cannabidiolic acid (CBDA), cannabinol (CBN), cannabichromene (CBC), cannabichromenic acid (CBCA), cannabigerol (CBG), c2022-01-01T00:00:00Z2022 May2025-04-04T20:36:06.657Z2025-04-04T20:36:06.657ZS-EPMC91039333556631410.3390/molecules27092965