biostudies-literatureZhang SThe National Natural Science Foundation of China/Research Grants Council Joint Research SchemeShenzhen Knowledge Innovation Programme of the Shenzhen Science and Technology Innovation CommissionThe Hong Kong University Shenzhen Hospital Scientific Research Training Plan4613https://www.ebi.ac.uk/biostudies/studies/S-EPMC9104239biostudies-literatureUnknown23(9)Human endometrium is an incredibly dynamic tissue undergoing cyclic regeneration and shedding during a woman's reproductive life. Endometrial mesenchymal stromal/stem-like cells (eMSC) contribute to this process. A hypoxic niche with low oxygen levels has been reported in multiple somatic stem cell types. However, the knowledge of hypoxia on eMSC remains limited. In mice, stromal stem/progenitor cells can be identified by the label-retaining technique. We examined the relationship between the label-retaining stromal cells (LRSC) and hypoxia during tissue breakdown in a mouse model of simulated menses. Our results demonstrated that LRSC resided in a hypoxic microenvironment during endometrial breakdown and early repair. Immunofluorescence staining revealed that the hypoxic-located LRSC undeInternational journal of molecular sciencesHypoxia Regulates the Self-Renewal of Endometrial Mesenchymal Stromal/Stem-like Cells via Notch Signaling.PMC9104239HKUSZH20192003JCYJ20180508153031952N_HKU 732/20Yeung WSBChan RWSNg EHYZhang SfalseHypoxia Regulates the Self-Renewal of Endometrial Mesenchymal Stromal/Stem-like Cells via Notch Signaling.Human endometrium is an incredibly dynamic tissue undergoing cyclic regeneration and shedding during a woman's reproductive life. Endometrial mesenchymal stromal/stem-like cells (eMSC) contribute to this process. A hypoxic niche with low oxygen levels has been reported in multiple somatic stem cell types. However, the knowledge of hypoxia on eMSC remains limited. In mice, stromal stem/progenitor cells can be identified by the label-retaining technique. We examined the relationship between the label-retaining stromal cells (LRSC) and hypoxia during tissue breakdown in a mouse model of simulated menses. Our results demonstrated that LRSC resided in a hypoxic microenvironment during endometrial breakdown and early repair. Immunofluorescence staining revealed that the hypoxic-located LRSC unde2022-01-01T00:00:00Z2022 Apr2025-04-04T20:35:59.909Z2025-04-04T20:35:59.909ZS-EPMC91042393556300310.3390/ijms23094613