biostudies-literatureAravindraja CNIDCR NIH HHS5107https://www.ebi.ac.uk/biostudies/studies/S-EPMC9105503biostudies-literatureUnknown23(9)Periodontitis (PD) is a polymicrobial dysbiotic immuno-inflammatory disease. It is more prevalent in males and has poorly understood pathogenic molecular mechanisms. Our primary objective was to characterize alterations in sex-specific microRNA (miRNA, miR) after periodontal bacterial infection. Using partial human mouth microbes (PAHMM) (<i>Streptococcus gordonii</i>, <i>Fusobacterium nucleatum</i>, <i>Porphyromonas gingivalis</i>, <i>Treponema denticola,</i> and <i>Tannerella forsythia</i>) in an ecological time-sequential polybacterial periodontal infection (ETSPPI) mouse model, we evaluated differential mandibular miRNA profiles by using high-throughput Nanostring nCounter^® miRNA expression panels. All PAHMM mice showed bacterial colonization (100%) in the gingival surface, International journal of molecular sciencesGlobal Noncoding microRNA Profiling in Mice Infected with Partial Human Mouth Microbes (PAHMM) Using an Ecological Time-Sequential Polybacterial Periodontal Infection (ETSPPI) Model Reveals Sex-Specific Differential microRNA Expression.PMC9105503R01 DE028536Chan EKLKesavalu LAravindraja CKashef MRGhanta RKVekariya KMKaranth SfalseGlobal Noncoding microRNA Profiling in Mice Infected with Partial Human Mouth Microbes (PAHMM) Using an Ecological Time-Sequential Polybacterial Periodontal Infection (ETSPPI) Model Reveals Sex-Specific Differential microRNA Expression.Periodontitis (PD) is a polymicrobial dysbiotic immuno-inflammatory disease. It is more prevalent in males and has poorly understood pathogenic molecular mechanisms. Our primary objective was to characterize alterations in sex-specific microRNA (miRNA, miR) after periodontal bacterial infection. Using partial human mouth microbes (PAHMM) (<i>Streptococcus gordonii</i>, <i>Fusobacterium nucleatum</i>, <i>Porphyromonas gingivalis</i>, <i>Treponema denticola,</i> and <i>Tannerella forsythia</i>) in an ecological time-sequential polybacterial periodontal infection (ETSPPI) mouse model, we evaluated differential mandibular miRNA profiles by using high-throughput Nanostring nCounter^® miRNA expression panels. All PAHMM mice showed bacterial colonization (100%) in the gingival surface, 2022-01-01T00:00:00Z2022 May2025-04-03T22:33:32.074Z2024-10-16T02:36:37.761ZS-EPMC91055033556350110.3390/ijms23095107