{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang S"],"funding":["the China Postdoctoral Science Foundation","the Guangdong Basic and Applied Basic Research Fund: Regional Joint Fund-Youth Fund Project"],"pagination":["4600"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9105514"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(9)"],"pubmed_abstract":["N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is the most common modification in eukaryotic RNAs. Accumulating evidence shows m<sup>6</sup>A methylation plays vital roles in various biological processes, including muscle and fat differentiation. However, there is a lack of research on lncRNAs' m<sup>6</sup>A modification in regulating pig muscle-fiber-type conversion. In this study, we identified novel and differentially expressed lncRNAs in oxidative and glycolytic skeletal muscles through RNA-seq, and further reported the m<sup>6</sup>A-methylation patterns of lncRNAs via MeRIP-seq. We found that most lncRNAs have one m<sup>6</sup>A peak, and the m<sup>6</sup>A peaks were preferentially enriched in the last exon of the lncRNAs. Interestingly, we found that lncRNAs' m<sup>6</sup>A levels were positively correlated with their expression homeostasis and levels. Furthermore, we performed conjoint analysis of MeRIP-seq and RNA-seq data and obtained 305 differentially expressed and differentially m<sup>6</sup>A-modified lncRNAs (dme-lncRNAs). Through QTL enrichment analysis of dme-lncRNAs and PPI analysis for their cis-genes, we finally identified seven key m<sup>6</sup>A-modified lncRNAs that may play a potential role in muscle-fiber-type conversion. Notably, inhibition of one of the key lncRNAs, <i>MSTRG.14200.1</i>, delayed satellite cell differentiation and stimulated fast-to-slow muscle-fiber conversion. Our study comprehensively analyzed m<sup>6</sup>A modifications on lncRNAs in oxidative and glycolytic skeletal muscles and provided new targets for the study of pig muscle-fiber-type conversion."],"journal":["International journal of molecular sciences"],"pubmed_title":["Comprehensive Analysis of Long Noncoding RNA Modified by m<sup>6</sup>A Methylation in Oxidative and Glycolytic Skeletal Muscles."],"pmcid":["PMC9105514"],"funding_grant_id":["2020A1515110319","2021M691075"],"pubmed_authors":["Hong L","Gu T","Wu Z","Yang J","Xiao L","Wang S","Zeng J","Tan B","Zheng E","Zhao X","Cai G"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive Analysis of Long Noncoding RNA Modified by m<sup>6</sup>A Methylation in Oxidative and Glycolytic Skeletal Muscles.","description":"N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is the most common modification in eukaryotic RNAs. Accumulating evidence shows m<sup>6</sup>A methylation plays vital roles in various biological processes, including muscle and fat differentiation. However, there is a lack of research on lncRNAs' m<sup>6</sup>A modification in regulating pig muscle-fiber-type conversion. In this study, we identified novel and differentially expressed lncRNAs in oxidative and glycolytic skeletal muscles through RNA-seq, and further reported the m<sup>6</sup>A-methylation patterns of lncRNAs via MeRIP-seq. We found that most lncRNAs have one m<sup>6</sup>A peak, and the m<sup>6</sup>A peaks were preferentially enriched in the last exon of the lncRNAs. Interestingly, we found that lncRNAs' m<sup>6</sup>A levels were positively correlated with their expression homeostasis and levels. Furthermore, we performed conjoint analysis of MeRIP-seq and RNA-seq data and obtained 305 differentially expressed and differentially m<sup>6</sup>A-modified lncRNAs (dme-lncRNAs). Through QTL enrichment analysis of dme-lncRNAs and PPI analysis for their cis-genes, we finally identified seven key m<sup>6</sup>A-modified lncRNAs that may play a potential role in muscle-fiber-type conversion. Notably, inhibition of one of the key lncRNAs, <i>MSTRG.14200.1</i>, delayed satellite cell differentiation and stimulated fast-to-slow muscle-fiber conversion. Our study comprehensively analyzed m<sup>6</sup>A modifications on lncRNAs in oxidative and glycolytic skeletal muscles and provided new targets for the study of pig muscle-fiber-type conversion.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-22T11:49:39.872Z","creation":"2025-02-19T03:41:46.46Z"},"accession":"S-EPMC9105514","cross_references":{"pubmed":["35562992"],"doi":["10.3390/ijms23094600"]}}