{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yuan XH"],"funding":["National Natural Science Foundation of China"],"pagination":["81"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9107719"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["<h4>Background</h4>Human rhinovirus C (HRV-C) accounts for a large proportion of HRV-related illnesses, but the immune response to HRV-C infection has not been elucidated. Our objective was to assess the effect of HRV-C on cytokine secretion in human bronchial epithelial (HBE) cells grown at air-liquid interface (ALI) and compare it with that of respiratory syncytial virus (RSV).<h4>Methods</h4>HBE cells were differentiated at ALI culture and the full-length cDNA clones of HRV-C651 and HRV-C15, clinical isolates of HRV-C79 and HRV-C101, and two RSV isolates were inoculated in the HBE cells. The effect of HRV-C on cytokine secretion was assessed and compared with that of RSV.<h4>Results</h4>HRV-Cs infect and propagate in fully differentiated HBE cells and significantly increase the secretion of IFN-λ1, CCL5, IP10, IL-6, IL-8, and MCP-1. The virus loads positively correlated with the levels of the cytokines. HRV-C induced lower secretion of CCL5 (P = 0.048), IL-6 (P = 0.016), MCP-1 (P = 0.008), and IL-8 (P = 0.032), and similar secretion of IP10 (P = 0.214) and IFN-λ1 (P = 0.214) when compared with RSV.<h4>Conclusion</h4>HBE ALI culture system supported HRV-C infection and propagation and HRV-C induced relatively weaker cytokine expression than RSV."],"journal":["Virology journal"],"pubmed_title":["Comparison of immune response to human rhinovirus C and respiratory syncytial virus in highly differentiated human airway epithelial cells."],"pmcid":["PMC9107719"],"funding_grant_id":["81672020"],"pubmed_authors":["Pang LL","Yang J","Yuan XH","Jin Y"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of immune response to human rhinovirus C and respiratory syncytial virus in highly differentiated human airway epithelial cells.","description":"<h4>Background</h4>Human rhinovirus C (HRV-C) accounts for a large proportion of HRV-related illnesses, but the immune response to HRV-C infection has not been elucidated. Our objective was to assess the effect of HRV-C on cytokine secretion in human bronchial epithelial (HBE) cells grown at air-liquid interface (ALI) and compare it with that of respiratory syncytial virus (RSV).<h4>Methods</h4>HBE cells were differentiated at ALI culture and the full-length cDNA clones of HRV-C651 and HRV-C15, clinical isolates of HRV-C79 and HRV-C101, and two RSV isolates were inoculated in the HBE cells. The effect of HRV-C on cytokine secretion was assessed and compared with that of RSV.<h4>Results</h4>HRV-Cs infect and propagate in fully differentiated HBE cells and significantly increase the secretion of IFN-λ1, CCL5, IP10, IL-6, IL-8, and MCP-1. The virus loads positively correlated with the levels of the cytokines. HRV-C induced lower secretion of CCL5 (P = 0.048), IL-6 (P = 0.016), MCP-1 (P = 0.008), and IL-8 (P = 0.032), and similar secretion of IP10 (P = 0.214) and IFN-λ1 (P = 0.214) when compared with RSV.<h4>Conclusion</h4>HBE ALI culture system supported HRV-C infection and propagation and HRV-C induced relatively weaker cytokine expression than RSV.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-04T19:31:42.5Z","creation":"2025-02-19T02:57:57.31Z"},"accession":"S-EPMC9107719","cross_references":{"pubmed":["35570279"],"doi":["10.1186/s12985-022-01805-2"]}}