{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["AbuSalim JE"],"funding":["National Cancer Institute","NCI NIH HHS","National Institutes of Health Intramural Research Program","Japan Society for the Promotion of Science"],"pagination":["2144-2150"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9107957"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(11)"],"pubmed_abstract":["Alpha-ketoglutarate (α-KG) is a key metabolite and signaling molecule in cancer cells, but the low permeability of α-KG limits the study of α-KG mediated effects in vivo. Recently, cell-permeable monoester and diester α-KG derivatives have been synthesized for use in vivo, but many of these derivatives are not compatible for use in hyperpolarized carbon-13 nuclear magnetic resonance spectroscopy (HP-<sup>13</sup>C-MRS). HP-<sup>13</sup>C-MRS is a powerful technique that has been used to noninvasively trace labeled metabolites in real time. Here, we show that using diethyl-[1-<sup>13</sup>C]-α-KG as a probe in HP-<sup>13</sup>C-MRS allows for noninvasive tracing of α-KG metabolism in vivo."],"journal":["ACS chemical biology"],"pubmed_title":["Simple Esterification of [1-<sup>13</sup>C]-Alpha-Ketoglutarate Enhances Membrane Permeability and Allows for Noninvasive Tracing of Glutamate and Glutamine Production."],"pmcid":["PMC9107957"],"funding_grant_id":["R00 CA222493","K99 CA222493","R00CA222493"],"pubmed_authors":["Blackman B","Yamamoto K","Swenson RE","Miura N","Brender JR","Krishna MC","AbuSalim JE","Mushti C","Camphausen KA","Seki T","Kesarwala AH"],"additional_accession":[]},"is_claimable":false,"name":"Simple Esterification of [1-<sup>13</sup>C]-Alpha-Ketoglutarate Enhances Membrane Permeability and Allows for Noninvasive Tracing of Glutamate and Glutamine Production.","description":"Alpha-ketoglutarate (α-KG) is a key metabolite and signaling molecule in cancer cells, but the low permeability of α-KG limits the study of α-KG mediated effects in vivo. Recently, cell-permeable monoester and diester α-KG derivatives have been synthesized for use in vivo, but many of these derivatives are not compatible for use in hyperpolarized carbon-13 nuclear magnetic resonance spectroscopy (HP-<sup>13</sup>C-MRS). HP-<sup>13</sup>C-MRS is a powerful technique that has been used to noninvasively trace labeled metabolites in real time. Here, we show that using diethyl-[1-<sup>13</sup>C]-α-KG as a probe in HP-<sup>13</sup>C-MRS allows for noninvasive tracing of α-KG metabolism in vivo.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-25T23:40:19.264Z","creation":"2025-04-06T09:24:50.51Z"},"accession":"S-EPMC9107957","cross_references":{"pubmed":["34554724"],"doi":["10.1021/acschembio.1c00561"]}}