{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cattin M"],"funding":["NIAID NIH HHS"],"pagination":["e202200061"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9108342"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(7)"],"pubmed_abstract":["Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs). Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM<sub>197</sub> neoglycoconjugates. We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors. Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors. Subsequent global deprotection generated the 3-aminopropyl oligosaccharide ligands. ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both manno"],"journal":["Chembiochem : a European journal of chemical biology"],"pubmed_title":["Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies."],"pmcid":["PMC9108342"],"funding_grant_id":["R01 AI134299"],"pubmed_authors":["Ng K","Cattin M","Blaukopf M","Bruxelle JF","Kosma P","Pantophlet R"],"additional_accession":[]},"is_claimable":false,"name":"Synthetic Neoglycoconjugates of Hepta- and Nonamannoside Ligands for Eliciting Oligomannose-Specific HIV-1-Neutralizing Antibodies.","description":"Oligomannose-type glycans on the spike protein of HIV-1 constitute relevant epitopes to elicit broadly neutralizing antibodies (bnAbs). Herein we describe an improved synthesis of α- and β-linked hepta- and nonamannosyl ligands that were subsequently converted into BSA and CRM<sub>197</sub> neoglycoconjugates. We assembled the ligands from anomeric 3-azidopropyl spacer glycosides from select 3-O-protected thiocresyl mannoside donors. Chain extensions were achieved using [4+3] or [4+5] block synthesis of thiocresyl and trichloroacetimidate glycosyl donors. Subsequent global deprotection generated the 3-aminopropyl oligosaccharide ligands. ELISA binding data obtained with the β-anomeric hepta- and nonamannosyl conjugates with a selection of HIV-1 bnAbs showed comparable binding of both manno","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-05T11:27:47.416Z","creation":"2025-04-05T11:27:47.416Z"},"accession":"S-EPMC9108342","cross_references":{"pubmed":["35104013"],"doi":["10.1002/cbic.202200061"]}}