biostudies-literaturePannkuk ELNIAID NIH HHSNational Cancer InstituteNCI NIH HHSDivision of Intramural Research, National Institute of Allergy and Infectious Diseases5145-5155https://www.ebi.ac.uk/biostudies/studies/S-EPMC9109602biostudies-literatureUnknown20(11)An important component of ionizing radiation (IR) exposure after a radiological incident may include low-dose rate (LDR) exposures either externally or internally, such as from ¹³⁷Cs deposition. In this study, a novel irradiation system, VAriable Dose-rate External ¹³⁷Cs irradiatoR (VADER), was used to expose male and female mice to a variable LDR irradiation over a 30 d time span to simulate fall-out-type exposures in addition to biofluid collection from a reference dose rate (0.8 Gy/min). Radiation markers were identified by untargeted metabolomics and random forests. Mice exposed to LDR exposures were successfully identified from control groups based on their urine and serum metabolite profiles. In addition to metabolites commonly perturbed after IR exposure, we idJournal of proteome researchBiofluid Metabolomics of Mice Exposed to External Low-Dose Rate Radiation in a Novel Irradiation System, the Variable Dose-Rate External ¹³⁷Cs Irradiator.PMC9109602U19 AI067773P30 CA051008P30CA051008Laiakis ECBrenner DJAmundson SAMorton SRGarty GYFornace AJPujol-Canadell MGirgis MGhandhi SAPannkuk ELfalseBiofluid Metabolomics of Mice Exposed to External Low-Dose Rate Radiation in a Novel Irradiation System, the Variable Dose-Rate External ¹³⁷Cs Irradiator.An important component of ionizing radiation (IR) exposure after a radiological incident may include low-dose rate (LDR) exposures either externally or internally, such as from ¹³⁷Cs deposition. In this study, a novel irradiation system, VAriable Dose-rate External ¹³⁷Cs irradiatoR (VADER), was used to expose male and female mice to a variable LDR irradiation over a 30 d time span to simulate fall-out-type exposures in addition to biofluid collection from a reference dose rate (0.8 Gy/min). Radiation markers were identified by untargeted metabolomics and random forests. Mice exposed to LDR exposures were successfully identified from control groups based on their urine and serum metabolite profiles. In addition to metabolites commonly perturbed after IR exposure, we id2021-01-01T00:00:00Z2021 Nov2025-04-04T07:45:23.532Z2025-04-04T07:45:23.532ZS-EPMC91096023458593110.1021/acs.jproteome.1c00638