{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chugh S"],"funding":["National Institute of Allergy and Infectious Diseases","NIAID NIH HHS","Michigan State University"],"pagination":["1031-1040"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9112674"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(5)"],"pubmed_abstract":["Bovine leukemia virus (BLV) is a C-type retrovirus of cattle that causes huge economic losses with high infection rates in the majority of countries worldwide. To develop an anti-BLV vaccine, we constructed a peptide conjugate using the envelope glycoprotein gp51-peptide epitope, a putative receptor-binding site. This highly antigenic peptide was covalently linked to a mutant bacteriophage carrier (mQβ) using two different linker strategies, isothiocyanate (NCS) and dinitrophenyl adipate. Both constructs elicited higher anti-BLV peptide IgG titers than the corresponding conjugate with keyhole limpet hemocyanin protein carrier (gold standard) in mice with the NCS linker strategy requiring less sample processing. The mQβ-gp51-peptide construct is the first BLV peptide-based vaccine candidate"],"journal":["ACS infectious diseases"],"pubmed_title":["Design and Synthesis of Bovine Leukemia Virus-Associated Peptide-Based Qβ Conjugate Eliciting Long-Lasting Neutralizing Antibodies in Mice."],"pmcid":["PMC9112674"],"funding_grant_id":["R01 AI146210","R01AI146210"],"pubmed_authors":["Yuzbasiyan-Gurkan V","Swenson C","Huang X","Chugh S"],"additional_accession":[]},"is_claimable":false,"name":"Design and Synthesis of Bovine Leukemia Virus-Associated Peptide-Based Qβ Conjugate Eliciting Long-Lasting Neutralizing Antibodies in Mice.","description":"Bovine leukemia virus (BLV) is a C-type retrovirus of cattle that causes huge economic losses with high infection rates in the majority of countries worldwide. To develop an anti-BLV vaccine, we constructed a peptide conjugate using the envelope glycoprotein gp51-peptide epitope, a putative receptor-binding site. This highly antigenic peptide was covalently linked to a mutant bacteriophage carrier (mQβ) using two different linker strategies, isothiocyanate (NCS) and dinitrophenyl adipate. Both constructs elicited higher anti-BLV peptide IgG titers than the corresponding conjugate with keyhole limpet hemocyanin protein carrier (gold standard) in mice with the NCS linker strategy requiring less sample processing. The mQβ-gp51-peptide construct is the first BLV peptide-based vaccine candidate","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-05-29T16:22:58.382Z","creation":"2025-05-29T16:22:58.382Z"},"accession":"S-EPMC9112674","cross_references":{"pubmed":["35482583"],"doi":["10.1021/acsinfecdis.2c00001"]}}