{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li L"],"funding":["NIAID NIH HHS","NIH HHS"],"pagination":["63"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9113379"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["<h4>Background</h4>Neutralizing antibodies are approved drugs to treat coronavirus disease-2019 (COVID-19) patients, yet mutations in severe acute respiratory syndrome coronavirus (SARS-CoV-2) variants may reduce the antibody neutralizing activity. New monoclonal antibodies (mAbs) and antibody remolding strategies are recalled in the battle with COVID-19 epidemic.<h4>Results</h4>We identified multiple mAbs from antibody phage display library made from COVID-19 patients and further characterized the R3P1-E4 clone, which effectively suppressed SARS-CoV-2 infection and rescued the lethal phenotype in mice infected with SARS-CoV-2. Crystal structural analysis not only explained why R3P1-E4 had selectively reduced binding and neutralizing activity to SARS-CoV-2 variants carrying K417 mutations,"],"journal":["Cell & bioscience"],"pubmed_title":["Antibody engineering improves neutralization activity against K417 spike mutant SARS-CoV-2 variants."],"pmcid":["PMC9113379"],"funding_grant_id":["AI158154","R01AI069120","AI140718","R01 AI140718"],"pubmed_authors":["Deng YQ","Duan J","Yang H","Li J","Zu S","Guo Y","Jiao P","Rao Z","Aliyari SR","Li L","Shi Y","Gao M","Cao Y","Cheng G","Wan D","Qin CF"],"additional_accession":[]},"is_claimable":false,"name":"Antibody engineering improves neutralization activity against K417 spike mutant SARS-CoV-2 variants.","description":"<h4>Background</h4>Neutralizing antibodies are approved drugs to treat coronavirus disease-2019 (COVID-19) patients, yet mutations in severe acute respiratory syndrome coronavirus (SARS-CoV-2) variants may reduce the antibody neutralizing activity. New monoclonal antibodies (mAbs) and antibody remolding strategies are recalled in the battle with COVID-19 epidemic.<h4>Results</h4>We identified multiple mAbs from antibody phage display library made from COVID-19 patients and further characterized the R3P1-E4 clone, which effectively suppressed SARS-CoV-2 infection and rescued the lethal phenotype in mice infected with SARS-CoV-2. Crystal structural analysis not only explained why R3P1-E4 had selectively reduced binding and neutralizing activity to SARS-CoV-2 variants carrying K417 mutations,","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2026-05-31T07:09:24.737Z","creation":"2025-02-19T05:05:31.65Z"},"accession":"S-EPMC9113379","cross_references":{"pubmed":["35581593"],"doi":["10.1186/s13578-022-00794-7"]}}