{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Trulsson F"],"funding":["Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","EC | Horizon 2020 Framework Programme","Statens Naturvidenskabelige Forskningsrad"],"pagination":["2736"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9117253"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Here, we compare the contributions of the proteasome and DUBs on the global ubiquitinome, using UbiSite technology, inhibitors and mass spectrometry. We uncover large dynamic ubiquitin signalling networks with substrates and sites preferentially regulated by DUBs or by the proteasome, highlighting the role of DUBs in degradation-independent ubiquitination. DUBs regulate substrates via at least 40,000 unique sites. Regulated networks of ubiquitin substrates are involved in autophagy, apoptosis, genome integrity, telomere integrity, cell cycle progression, mitochondrial function, vesicle transport, signal transducti"],"journal":["Nature communications"],"pubmed_title":["Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates."],"pmcid":["PMC9117253"],"funding_grant_id":["765445","141","RGPIN 2016-05868"],"pubmed_authors":["Vertegaal ACO","Berrocal DAP","Cox J","Akimov V","Trulsson F","Shah GM","Robu M","Shah RG","Blagoev B","van Overbeek N"],"additional_accession":[]},"is_claimable":false,"name":"Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates.","description":"The ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Here, we compare the contributions of the proteasome and DUBs on the global ubiquitinome, using UbiSite technology, inhibitors and mass spectrometry. We uncover large dynamic ubiquitin signalling networks with substrates and sites preferentially regulated by DUBs or by the proteasome, highlighting the role of DUBs in degradation-independent ubiquitination. DUBs regulate substrates via at least 40,000 unique sites. Regulated networks of ubiquitin substrates are involved in autophagy, apoptosis, genome integrity, telomere integrity, cell cycle progression, mitochondrial function, vesicle transport, signal transducti","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-05-29T22:27:27.482Z","creation":"2025-05-29T22:27:27.482Z"},"accession":"S-EPMC9117253","cross_references":{"pubmed":["35585066"],"doi":["10.1038/s41467-022-30376-7"]}}