biostudies-literatureKosuri SNational Institutes of HealthNIGMS NIH HHSNew Jersey Commission on Spinal Cord ResearchNational Science Foundatione2102101https://www.ebi.ac.uk/biostudies/studies/S-EPMC9119153biostudies-literatureUnknown11(10)Among the many molecules that contribute to glial scarring, chondroitin sulfate proteoglycans (CSPGs) are known to be potent inhibitors of neuronal regeneration. Chondroitinase ABC (ChABC), a bacterial lyase, degrades the glycosaminoglycan (GAG) side chains of CSPGs and promotes tissue regeneration. However, ChABC is thermally unstable and loses all activity within a few hours at 37 °C under dilute conditions. To overcome this limitation, the discovery of a diverse set of tailor-made random copolymers that complex and stabilize ChABC at physiological temperature is reported. The copolymer designs, which are based on chain length and composition of the copolymers, are identified using an active machine learning paradigm, which involves iterative copolymer synthesis, testing for ChABC thermoAdvanced healthcare materialsMachine-Assisted Discovery of Chondroitinase ABC Complexes toward Sustained Neural Regeneration.PMC9119153NIGMS R35GM138296T32 GM135141NSF‐DMR‐2118861R35 GM138296NSF‐DMR‐2118860CSCR18IRG007Mugnier HSchloss RGormley AJTamasi MPatel RAFinkel ZCai LBorca CHKumar SWebb MAYarmush MLPerez IKosuri SfalseMachine-Assisted Discovery of Chondroitinase ABC Complexes toward Sustained Neural Regeneration.Among the many molecules that contribute to glial scarring, chondroitin sulfate proteoglycans (CSPGs) are known to be potent inhibitors of neuronal regeneration. Chondroitinase ABC (ChABC), a bacterial lyase, degrades the glycosaminoglycan (GAG) side chains of CSPGs and promotes tissue regeneration. However, ChABC is thermally unstable and loses all activity within a few hours at 37 °C under dilute conditions. To overcome this limitation, the discovery of a diverse set of tailor-made random copolymers that complex and stabilize ChABC at physiological temperature is reported. The copolymer designs, which are based on chain length and composition of the copolymers, are identified using an active machine learning paradigm, which involves iterative copolymer synthesis, testing for ChABC thermo2022-01-01T00:00:00Z2022 May2025-05-29T16:22:23.095Z2025-05-29T16:22:23.095ZS-EPMC91191533511250810.1002/adhm.202102101