{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Luyten YA"],"funding":["G. Harold and Leila Y. Mathers Charitable Foundation","US Department of Energy","Howard Hughes Medical Institute","Fred Hutchinson Cancer Research Center","New England Biolabs","National Institute of General Medical Sciences","NIGMS NIH HHS","NIH HHS"],"pagination":["5171-5190"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9122589"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(9)"],"pubmed_abstract":["Bacteriophage exclusion ('BREX') phage restriction systems are found in a wide range of bacteria. Various BREX systems encode unique combinations of proteins that usually include a site-specific methyltransferase; none appear to contain a nuclease. Here we describe the identification and characterization of a Type I BREX system from Acinetobacter and the effect of deleting each BREX ORF on growth, methylation, and restriction. We identified a previously uncharacterized gene in the BREX operon that is dispensable for methylation but involved in restriction. Biochemical and crystallographic analyses of this factor, which we term BrxR ('BREX Regulator'), demonstrate that it forms a homodimer and specifically binds a DNA target site upstream of its transcription start site. Deletion of the Brx"],"journal":["Nucleic acids research"],"pubmed_title":["Identification and characterization of the WYL BrxR protein and its gene as separable regulatory elements of a BREX phage restriction system."],"pmcid":["PMC9122589"],"funding_grant_id":["DE-AC02-05CH11231","R15 GM140375","R01 GM105691","P30 GM124169","S10 OD021832"],"pubmed_authors":["Doyle LA","Young JC","Kaiser BK","Ubilla-Rodriguez NC","Morgan RD","Lambert AR","Luyten YA","Hausman DE","Higashi KM","Stoddard BL","Arroyo CS","Forsberg KJ"],"additional_accession":[]},"is_claimable":false,"name":"Identification and characterization of the WYL BrxR protein and its gene as separable regulatory elements of a BREX phage restriction system.","description":"Bacteriophage exclusion ('BREX') phage restriction systems are found in a wide range of bacteria. Various BREX systems encode unique combinations of proteins that usually include a site-specific methyltransferase; none appear to contain a nuclease. Here we describe the identification and characterization of a Type I BREX system from Acinetobacter and the effect of deleting each BREX ORF on growth, methylation, and restriction. We identified a previously uncharacterized gene in the BREX operon that is dispensable for methylation but involved in restriction. Biochemical and crystallographic analyses of this factor, which we term BrxR ('BREX Regulator'), demonstrate that it forms a homodimer and specifically binds a DNA target site upstream of its transcription start site. Deletion of the Brx","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2026-04-08T17:52:16.84Z","creation":"2025-02-19T01:54:39.323Z"},"accession":"S-EPMC9122589","cross_references":{"pubmed":["35511079"],"doi":["10.1093/nar/gkac311"]}}