{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Klask CM"],"pubmed_abstract":["For <i>Clostridium ljungdahlii</i>, the RNF complex plays a key role for energy conversion from gaseous substrates such as hydrogen and carbon dioxide. In a previous study, a disruption of RNF-complex genes led to the loss of autotrophy, while heterotrophy was still possible <i>via</i> glycolysis. Furthermore, it was shown that the energy limitation during autotrophy could be lifted by nitrate supplementation, which resulted in an elevated cellular growth and ATP yield. Here, we used CRISPR-Cas12a to delete: <b>(1)</b> the RNF complex-encoding gene cluster <i>rnfCDGEAB</i>; <b>(2)</b> the putative RNF regulator gene <i>rseC</i>; and <b>(3)</b> a gene cluster that encodes for a putative nitrate reductase. The deletion of either <i>rnfCDGEAB</i> or <i>rseC</i> resulted in a complete loss of "],"journal":["Frontiers in microbiology"],"pagination":["887578"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9124969"],"repository":["biostudies-literature"],"pubmed_title":["Genetic Evidence Reveals the Indispensable Role of the <i>rseC</i> Gene for Autotrophy and the Importance of a Functional Electron Balance for Nitrate Reduction in <i>Clostridium ljungdahlii</i>."],"pmcid":["PMC9124969"],"pubmed_authors":["Jager B","Klask CM","Molitor B","Angenent LT","Casini I"],"additional_accession":[]},"is_claimable":false,"name":"Genetic Evidence Reveals the Indispensable Role of the <i>rseC</i> Gene for Autotrophy and the Importance of a Functional Electron Balance for Nitrate Reduction in <i>Clostridium ljungdahlii</i>.","description":"For <i>Clostridium ljungdahlii</i>, the RNF complex plays a key role for energy conversion from gaseous substrates such as hydrogen and carbon dioxide. In a previous study, a disruption of RNF-complex genes led to the loss of autotrophy, while heterotrophy was still possible <i>via</i> glycolysis. Furthermore, it was shown that the energy limitation during autotrophy could be lifted by nitrate supplementation, which resulted in an elevated cellular growth and ATP yield. Here, we used CRISPR-Cas12a to delete: <b>(1)</b> the RNF complex-encoding gene cluster <i>rnfCDGEAB</i>; <b>(2)</b> the putative RNF regulator gene <i>rseC</i>; and <b>(3)</b> a gene cluster that encodes for a putative nitrate reductase. The deletion of either <i>rnfCDGEAB</i> or <i>rseC</i> resulted in a complete loss of ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-05-29T21:53:17.092Z","creation":"2024-11-09T04:33:48.542Z"},"accession":"S-EPMC9124969","cross_references":{"pubmed":["35615511"],"doi":["10.3389/fmicb.2022.887578"]}}