biostudies-literatureLu QLocal Science and Technology Program of Shaanxi ProvinceNatural Science Basic Research Program of Shaanxi8734https://www.ebi.ac.uk/biostudies/studies/S-EPMC9130509biostudies-literatureUnknown12(1)Lignin, a highly polymerized organic component of plant cells, is one of the most difficult aromatic substances to degrade. Selective biodegradation under mild conditions is a promising method, but the dynamic variations in lignin monomers during the biodegradation of lignocellulose are not fully understood. In this study, we evaluated the differences in lignin degradation under different microbial inoculation based on the lignin monomer content, monomer ratio, and stable hydrogen isotope ratio of lignin methoxy groups (δ²H_LM). The weight loss during degradation and the net loss of lignocellulosic components improved dramatically with fungal inoculation. Syringyl monolignol (S-lignin), which contains two methoxy groups, was more difficult to degrade than guaiacyl (G-lScientific reportsVariations in lignin monomer contents and stable hydrogen isotope ratios in methoxy groups during the biodegradation of garden biomass.PMC91305092021JQ-9682021ZDYF-GY-00202020JM-7082020JQ-971Zhao NChen ZZhang ZHe LWang YJia LLu QAwasthi MKJing GShi XfalseVariations in lignin monomer contents and stable hydrogen isotope ratios in methoxy groups during the biodegradation of garden biomass.Lignin, a highly polymerized organic component of plant cells, is one of the most difficult aromatic substances to degrade. Selective biodegradation under mild conditions is a promising method, but the dynamic variations in lignin monomers during the biodegradation of lignocellulose are not fully understood. In this study, we evaluated the differences in lignin degradation under different microbial inoculation based on the lignin monomer content, monomer ratio, and stable hydrogen isotope ratio of lignin methoxy groups (δ²H_LM). The weight loss during degradation and the net loss of lignocellulosic components improved dramatically with fungal inoculation. Syringyl monolignol (S-lignin), which contains two methoxy groups, was more difficult to degrade than guaiacyl (G-l2022-01-01T00:00:00Z2022 May2025-04-04T22:15:06.102Z2024-11-13T06:12:45.949ZS-EPMC91305093561035410.1038/s41598-022-12689-1