biostudies-literatureUnknown13Li BIonizing radiation (IR) is an effective approach for mutation breeding. Understanding the mutagenesis and transcriptional profiles induced by different mutagens is of great significance for improving mutation breeding efficiency. Here, using RNA sequencing and methylation-sensitive amplification polymorphism (MSAP) approaches, we compared the genetic variations, epigenetics, and transcriptional responses induced by the mixed high-energy particle field (CR) and ⁷Li-ion beam (LR) radiation in M₁ seedlings of two wheat genotypes (Yangmai 18 and Yangmai 20). The results showed that, in both wheat genotypes, CR displayed significantly a higher mutation efficiency (1.79 × 10^-6/bp) than that by LR (1.56 × 10^-6/bp). The induced mutations were not evenly Frontiers in plant science878420https://www.ebi.ac.uk/biostudies/studies/S-EPMC9131052biostudies-literatureThe Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and ⁷Li-Ion Beams (LR) Radiation in Wheat M₁ Seedlings.PMC9131052Xu LAn BZhao LHe YLiu LWang RLi BJiao CZhang SCai HLiu GXu YfalseThe Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and ⁷Li-Ion Beams (LR) Radiation in Wheat M₁ Seedlings.Ionizing radiation (IR) is an effective approach for mutation breeding. Understanding the mutagenesis and transcriptional profiles induced by different mutagens is of great significance for improving mutation breeding efficiency. Here, using RNA sequencing and methylation-sensitive amplification polymorphism (MSAP) approaches, we compared the genetic variations, epigenetics, and transcriptional responses induced by the mixed high-energy particle field (CR) and ⁷Li-ion beam (LR) radiation in M₁ seedlings of two wheat genotypes (Yangmai 18 and Yangmai 20). The results showed that, in both wheat genotypes, CR displayed significantly a higher mutation efficiency (1.79 × 10^-6/bp) than that by LR (1.56 × 10^-6/bp). The induced mutations were not evenly 2022-01-01T00:00:00Z20222026-04-08T09:50:59.841Z2024-11-10T08:43:46.574ZS-EPMC91310523564603310.3389/fpls.2022.878420