biostudies-literatureNaven MAVersus ArthritisMedical Research CouncilBiotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research Council3963-3976https://www.ebi.ac.uk/biostudies/studies/S-EPMC9131279biostudies-literatureUnknown12(8)The circadian clock in murine articular cartilage is a critical temporal regulatory mechanism for tissue homeostasis and osteoarthritis. However, translation of these findings into humans has been hampered by the difficulty in obtaining circadian time series human cartilage tissues. As such, a suitable model is needed to understand the initiation and regulation of circadian rhythms in human cartilage. <b>Methods:</b> We used a chondrogenic differentiation protocol on human embryonic stem cells (hESCs) as a proxy for early human chondrocyte development. Chondrogenesis was validated using histology and expression of pluripotency and differentiation markers. The molecular circadian clock was tracked in real time by lentiviral transduction of human clock gene luciferase reporters. DifferentiatTheranosticsDevelopment of human cartilage circadian rhythm in a stem cell-chondrogenesis model.PMC9131279R20786MR/T016744/1BB/W013940/1MR/P010709/1MR/L004992/1MR/L011840/1MR/S002553/1G0801057179175620875Pathiranage DBates NHumphreys PACain SWoods SSmith CALi SZeef LAHWen CKimber SJNaven MAAu MMeng QJfalseDevelopment of human cartilage circadian rhythm in a stem cell-chondrogenesis model.The circadian clock in murine articular cartilage is a critical temporal regulatory mechanism for tissue homeostasis and osteoarthritis. However, translation of these findings into humans has been hampered by the difficulty in obtaining circadian time series human cartilage tissues. As such, a suitable model is needed to understand the initiation and regulation of circadian rhythms in human cartilage. <b>Methods:</b> We used a chondrogenic differentiation protocol on human embryonic stem cells (hESCs) as a proxy for early human chondrocyte development. Chondrogenesis was validated using histology and expression of pluripotency and differentiation markers. The molecular circadian clock was tracked in real time by lentiviral transduction of human clock gene luciferase reporters. Differentiat2022-01-01T00:00:00Z20222026-05-09T16:39:15.649Z2024-11-15T12:36:00.955ZS-EPMC91312793566407210.7150/thno.70893