biostudies-literatureUnknown12Li XNational Natural Science Foundation of China<h4>Objectives</h4>To characterize two plasmids co-harboring carbapenem resistance genes and <i>tmexCD2-toprJ2</i> in carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) strains.<h4>Methods</h4>Two clinical CRKP strains were isolated and characterized by antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, and bioinformatics analysis.<h4>Results</h4>The two CRKP strains NB4 and NB5 were both resistant to imipenem, meropenem and tigecycline. Whole-genome sequencing revealed that two CRKP strains belonged to the ST11 type and carried multiple resistance genes. The <i>tmexCD2-toprJ2</i> clusters in both strains were located on the IncFIB(Mar)-like/HI1B-like group of hybrid plasmids, which co-harbored the metallo-β-lactamase gene <i>bla</i>_NDM-1. IFrontiers in cellular and infection microbiology902774https://www.ebi.ac.uk/biostudies/studies/S-EPMC9134201biostudies-literatureEmergence of Plasmids Co-Harboring Carbapenem Resistance Genes and <i>tmexCD2-toprJ2</i> in Sequence Type 11 Carbapenem Resistant <i>Klebsiella pneumoniae</i> Strains.PMC9134201Zou XLi XMa QJin XZhang XHuang HWang WHu QTu YfalseEmergence of Plasmids Co-Harboring Carbapenem Resistance Genes and <i>tmexCD2-toprJ2</i> in Sequence Type 11 Carbapenem Resistant <i>Klebsiella pneumoniae</i> Strains.<h4>Objectives</h4>To characterize two plasmids co-harboring carbapenem resistance genes and <i>tmexCD2-toprJ2</i> in carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) strains.<h4>Methods</h4>Two clinical CRKP strains were isolated and characterized by antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, and bioinformatics analysis.<h4>Results</h4>The two CRKP strains NB4 and NB5 were both resistant to imipenem, meropenem and tigecycline. Whole-genome sequencing revealed that two CRKP strains belonged to the ST11 type and carried multiple resistance genes. The <i>tmexCD2-toprJ2</i> clusters in both strains were located on the IncFIB(Mar)-like/HI1B-like group of hybrid plasmids, which co-harbored the metallo-β-lactamase gene <i>bla</i>_NDM-1. I2022-01-01T00:00:00Z20222026-05-09T19:51:57.97Z2024-11-20T23:04:20.316ZS-EPMC91342013564674010.3389/fcimb.2022.902774