biostudies-literatureKuroyanagi GGovernment of Japan Ministry of Education Culture Sports Science and TechnologyNational Center for Geriatrics and Gerontology495https://www.ebi.ac.uk/biostudies/studies/S-EPMC9134601biostudies-literatureUnknown23(1)<h4>Background</h4>Heat shock protein (HSP) 90 functions as a molecular chaperone and is constitutively expressed and induced in response to stress in many cell types. We have previously demonstrated that transforming growth factor-β (TGF-β), the most abundant cytokine in bone cells, induces the expression of HSP27 through Smad2, p44/p42 mitogen-activated protein kinase (MAPK), p38 MAPK, and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in mouse osteoblastic MC3T3-E1 cells. This study investigated the effects of HSP90 on the TGF-β-induced HSP27 expression and the underlying mechanism in mouse osteoblastic MC3T3-E1 cells.<h4>Methods</h4>Clonal osteoblastic MC3T3-E1 cells were treated with the HSP90 inhibitors and then stimulated with TGF-β. HSP27 expression and the phosBMC musculoskeletal disordersUpregulation of TGF-β-induced HSP27 by HSP90 inhibitors in osteoblasts.PMC913460119K1847115K10487 and 17K1100228-9 and 29-12Sakai GKawabata TKuroyanagi GKim WTachi JTokuda HMatsushima-Nishiwaki RHioki TFujita KIida HKozawa OOtsuka TfalseUpregulation of TGF-β-induced HSP27 by HSP90 inhibitors in osteoblasts.<h4>Background</h4>Heat shock protein (HSP) 90 functions as a molecular chaperone and is constitutively expressed and induced in response to stress in many cell types. We have previously demonstrated that transforming growth factor-β (TGF-β), the most abundant cytokine in bone cells, induces the expression of HSP27 through Smad2, p44/p42 mitogen-activated protein kinase (MAPK), p38 MAPK, and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) in mouse osteoblastic MC3T3-E1 cells. This study investigated the effects of HSP90 on the TGF-β-induced HSP27 expression and the underlying mechanism in mouse osteoblastic MC3T3-E1 cells.<h4>Methods</h4>Clonal osteoblastic MC3T3-E1 cells were treated with the HSP90 inhibitors and then stimulated with TGF-β. HSP27 expression and the phos2022-01-01T00:00:00Z2022 May2025-04-26T02:38:54.065Z2025-02-19T02:16:21.138ZS-EPMC91346013561909410.1186/s12891-022-05419-1