{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Heim M"],"funding":["Agence Nationale de la Recherche","EU Joint Programme – Neurodegenerative Disease Research","Centre National de la Recherche Scientifique"],"pagination":["101415"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9136351"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(2)"],"pubmed_abstract":["RNA-binding proteins (RBPs) are multifunctional proteins that shuttle between the nucleus and the cytoplasm where they assemble with target RNAs to form multi-molecular complexes. Here, we describe a protocol to selectively identify RNAs associated with RBPs of interest in the cytoplasmic and nuclear compartments of adult <i>Drosophila</i> brain cells. Cytoplasmic and nuclear fractions are differentially collected and used for immunoprecipitation-based purification of GFP-tagged RBPs. This protocol can be applied to samples expressing ectopic or endogenous tagged RBPs."],"journal":["STAR protocols"],"pubmed_title":["An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclear <i>Drosophila</i> head fractions."],"pmcid":["PMC9136351"],"funding_grant_id":["ANR-18-CE13-0020-02"],"pubmed_authors":["Heim M","Besse F","Blot L"],"additional_accession":[]},"is_claimable":false,"name":"An RNA-immunoprecipitation protocol to identify RNAs associated with RNA-binding proteins in cytoplasmic and nuclear <i>Drosophila</i> head fractions.","description":"RNA-binding proteins (RBPs) are multifunctional proteins that shuttle between the nucleus and the cytoplasm where they assemble with target RNAs to form multi-molecular complexes. Here, we describe a protocol to selectively identify RNAs associated with RBPs of interest in the cytoplasmic and nuclear compartments of adult <i>Drosophila</i> brain cells. Cytoplasmic and nuclear fractions are differentially collected and used for immunoprecipitation-based purification of GFP-tagged RBPs. This protocol can be applied to samples expressing ectopic or endogenous tagged RBPs.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2026-05-09T21:54:58.234Z","creation":"2025-04-04T13:30:13.463Z"},"accession":"S-EPMC9136351","cross_references":{"pubmed":["35634357"],"doi":["10.1016/j.xpro.2022.101415"]}}