biostudies-literatureRen XBeijing Municipal Education CommissionNational Natural Science Foundation of ChinaBeijing Municipal Commission of Educatione13230https://www.ebi.ac.uk/biostudies/studies/S-EPMC9136491biostudies-literatureUnknown55(5)Stem cells maintain adult tissue homeostasis under physiological conditions. Uncontrolled stem cell proliferation will lead to tumorigenesis. How stem cell proliferation is precisely controlled is still not fully understood. Phosphorylation of Yun is essential for ISC proliferation. Yun is essential for the proliferation of normal and transformed intestinal stem cells. Our mass spectrometry and biochemical data suggest that Yun can be phosphorylated at multiple residues in vivo. Interestingly, we show that the phosphorylation among these residues is likely interdependent. Furthermore, phosphorylation of each residue in Yun is important for its function in ISC proliferation regulation. Thus, our study unveils the important role of post-translational modification of Yun in stem cell proliferCell proliferationPhosphorylation of Yun is required for stem cell proliferation and tumorigenesis.PMC91364919205410931471384KZ20191002804031972893Ren XShi LMa RLi ZKong RLu SWang JHWang YJia LZhao HDong MQfalsePhosphorylation of Yun is required for stem cell proliferation and tumorigenesis.Stem cells maintain adult tissue homeostasis under physiological conditions. Uncontrolled stem cell proliferation will lead to tumorigenesis. How stem cell proliferation is precisely controlled is still not fully understood. Phosphorylation of Yun is essential for ISC proliferation. Yun is essential for the proliferation of normal and transformed intestinal stem cells. Our mass spectrometry and biochemical data suggest that Yun can be phosphorylated at multiple residues in vivo. Interestingly, we show that the phosphorylation among these residues is likely interdependent. Furthermore, phosphorylation of each residue in Yun is important for its function in ISC proliferation regulation. Thus, our study unveils the important role of post-translational modification of Yun in stem cell prolifer2022-01-01T00:00:00Z2022 May2025-04-18T21:51:19.174Z2025-04-07T09:39:00.237ZS-EPMC91364913543786410.1111/cpr.13230