biostudies-literatureBalta IEnvirontech974https://www.ebi.ac.uk/biostudies/studies/S-EPMC9137680biostudies-literatureUnknown11(5)Nematopsis messor infections severely impact on shrimp’s health with devastating economic consequences on shrimp farming. In a shrimp primary intestinal cells (SGP) model of infection, a sub-inhibitory concentration (0.5%) of natural antimicrobials (Aq) was able to reduce the ability of N. messor to infect (p < 0.0001). To prevent N. messor infection of SGP cells, Aq inhibits host actin polymerization and restores tight junction integrity (TEER) and the expression of Zo-1 and occluding. The oxidative burst, caused by N. messor infection, is attenuated by Aq through the inhibition of NADPH-produced H2O2. Simultaneous to the reduction in H2O2 released, the activity of catalase (CAT) and superoxide dismutase (SOD) were also significantly increase (p < 0.0001). The antimicrobial mixture inactiAntioxidants (Basel, Switzerland)The Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected with <i>Nematopsis messor</i>.PMC913768049560Gundogdu OBalta IWard PVenig AButucel EStef DCorcionivoschi NKoyun OYCallaway TRDeshaies MStef LPet IGradisteanu Pircalabioru GfalseThe Antioxidant Effect of Natural Antimicrobials in Shrimp Primary Intestinal Cells Infected with <i>Nematopsis messor</i>.Nematopsis messor infections severely impact on shrimp’s health with devastating economic consequences on shrimp farming. In a shrimp primary intestinal cells (SGP) model of infection, a sub-inhibitory concentration (0.5%) of natural antimicrobials (Aq) was able to reduce the ability of N. messor to infect (p < 0.0001). To prevent N. messor infection of SGP cells, Aq inhibits host actin polymerization and restores tight junction integrity (TEER) and the expression of Zo-1 and occluding. The oxidative burst, caused by N. messor infection, is attenuated by Aq through the inhibition of NADPH-produced H2O2. Simultaneous to the reduction in H2O2 released, the activity of catalase (CAT) and superoxide dismutase (SOD) were also significantly increase (p < 0.0001). The antimicrobial mixture inacti2022-01-01T00:00:00Z2022 May2025-04-25T16:57:35.37Z2024-11-05T17:01:54.733ZS-EPMC91376803562483810.3390/antiox11050974