biostudies-literatureCaballero EJunta de Castilla y LeónMinisterio de Ciencia e Innovación, SpainJunta de Castilla y León, Spain1153https://www.ebi.ac.uk/biostudies/studies/S-EPMC9138537biostudies-literatureUnknown10(5)The molecular basis of amyloid toxicity in Alzheimer's disease (AD) remains controversial. Amyloid β (Aβ) oligomers promote Ca²⁺ influx, mitochondrial Ca²⁺ overload and apoptosis in hippocampal neurons in vivo and in vitro, but the primary Ca²⁺ entry pathways are unclear. We studied Ca²⁺ entry pathways induced by Aβ oligomers in rat hippocampal and cerebellar neurons. Aβ oligomers induce Ca²⁺ entry in neurons. Ca²⁺ responses to Aβ oligomers are large after synaptic networking and prevented by blockers of synaptic transmission. In contrast, in neurons devoid of synaptic connections, Ca²⁺ responses to Aβ oligomers are small and prevented only by blockers of amyloid channels (NA7) and NMDA receptors (MK801). A combinBiomedicinesAmyloid Beta Oligomers-Induced Ca²⁺ Entry Pathways: Role of Neuronal Networks, NMDA Receptors and Amyloid Channel Formation.PMC9138537CCVC8485VA294P18RTI2018-099298-B-10Nunez LVillalobos CHernando-Perez ECalvo-Rodriguez MTapias VCaballero EfalseAmyloid Beta Oligomers-Induced Ca²⁺ Entry Pathways: Role of Neuronal Networks, NMDA Receptors and Amyloid Channel Formation.The molecular basis of amyloid toxicity in Alzheimer's disease (AD) remains controversial. Amyloid β (Aβ) oligomers promote Ca²⁺ influx, mitochondrial Ca²⁺ overload and apoptosis in hippocampal neurons in vivo and in vitro, but the primary Ca²⁺ entry pathways are unclear. We studied Ca²⁺ entry pathways induced by Aβ oligomers in rat hippocampal and cerebellar neurons. Aβ oligomers induce Ca²⁺ entry in neurons. Ca²⁺ responses to Aβ oligomers are large after synaptic networking and prevented by blockers of synaptic transmission. In contrast, in neurons devoid of synaptic connections, Ca²⁺ responses to Aβ oligomers are small and prevented only by blockers of amyloid channels (NA7) and NMDA receptors (MK801). A combin2022-01-01T00:00:00Z2022 May2025-04-21T17:32:32.489Z2025-04-05T16:40:23.722ZS-EPMC91385373562589010.3390/biomedicines10051153