biostudies-literatureHahn LDeutsche Forschungsgemeinschaft707https://www.ebi.ac.uk/biostudies/studies/S-EPMC9139023biostudies-literatureUnknown12(5)Preeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet completely understood and without curative therapy. The histone modifications H3K4me³ and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of the development of PE, the influence of Gal-2 on histone modification in trophoblasts and in syncytialisation was investigated. Immunohistochemical stains of 13 PE and 13 control placentas were correlated, followed by cell culture experiments. An analysis of H3K4me³ and H3K9ac was conducted, as well as cell fusion staining with E-cadherin and β-catenin-both after incubation with Gal-2. The expression of H3K4me³ and H3K9ac correlated significantly with the expression of Gal-2. FurthermorBiomoleculesGal-2 Increases H3K4me³ and H3K9ac in Trophoblasts and Preeclampsia.PMC9139023413635475Corradini SMahner SJeschke UMeister SBeyer SHasbargen UMannewitz MKolben TBurges AHahn LfalseGal-2 Increases H3K4me³ and H3K9ac in Trophoblasts and Preeclampsia.Preeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet completely understood and without curative therapy. The histone modifications H3K4me³ and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of the development of PE, the influence of Gal-2 on histone modification in trophoblasts and in syncytialisation was investigated. Immunohistochemical stains of 13 PE and 13 control placentas were correlated, followed by cell culture experiments. An analysis of H3K4me³ and H3K9ac was conducted, as well as cell fusion staining with E-cadherin and β-catenin-both after incubation with Gal-2. The expression of H3K4me³ and H3K9ac correlated significantly with the expression of Gal-2. Furthermor2022-01-01T00:00:00Z2022 May2025-04-05T11:58:39.16Z2025-04-05T11:58:39.16ZS-EPMC91390233562563410.3390/biom12050707