biostudies-literatureSukenikova LCharles UniversityMinistry of Education, Youth and Sport of the Czech RepublicGrant Agency of Charles University1624https://www.ebi.ac.uk/biostudies/studies/S-EPMC9140140biostudies-literatureUnknown11(10)Early postnatal events are important for the development of the neonatal immune system. Harboring the pioneering microorganisms forming the microbiota of the neonatal gastrointestinal tract is important for priming the immune system, as well as inducing appropriate tolerance to the relatively innocuous environmental antigens and compounds of normal healthy microbiota. Early postnatal supplementation of suitable, safe probiotics could accelerate this process. In the current study, the immunomodulatory capacity of the probiotic strain of <i>Escherichia coli</i> O83:K24:H31 (EcO83) was characterized in vitro and in vivo. We compared the capacity of EcO83 with and without hemolytic activity on selected immune characteristics in vitro as determined by flow cytometry and quantitative real-time PCellsThe Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo.PMC9140140Cooperatio IMMU 207032GAUK 4781198J19AT010Progres Q25/LF1Petraskova PCerny VProkesova LVecek JNovotna OVobruba SKolarova LSukenikova LMichalcikova THrdy JProchazka JfalseThe Impact of <i>Escherichia coli</i> Probiotic Strain O83:K24:H31 on the Maturation of Dendritic Cells and Immunoregulatory Functions In Vitro and In Vivo.Early postnatal events are important for the development of the neonatal immune system. Harboring the pioneering microorganisms forming the microbiota of the neonatal gastrointestinal tract is important for priming the immune system, as well as inducing appropriate tolerance to the relatively innocuous environmental antigens and compounds of normal healthy microbiota. Early postnatal supplementation of suitable, safe probiotics could accelerate this process. In the current study, the immunomodulatory capacity of the probiotic strain of <i>Escherichia coli</i> O83:K24:H31 (EcO83) was characterized in vitro and in vivo. We compared the capacity of EcO83 with and without hemolytic activity on selected immune characteristics in vitro as determined by flow cytometry and quantitative real-time P2022-01-01T00:00:00Z2022 May2025-04-26T02:51:19.519Z2025-02-19T02:16:02.982ZS-EPMC91401403562666010.3390/cells11101624