biostudies-literatureVlasceanu GMNational Authority for Scientific Research and Innovation5336https://www.ebi.ac.uk/biostudies/studies/S-EPMC9140409biostudies-literatureUnknown23(10)We designed graphene oxide composites with increased morphological and structural variability using fatty acid-coupled polysaccharide co-polymer as the continuous phase. The matrix was synthesized by N, O-acylation of chitosan with palmitic and lauric acid. The obtained co-polymer was crosslinked with genipin and composited with graphene oxide. FTIR spectra highlighted the modification and multi-components interaction. DLS, SEM, and contact angle tests demonstrated that the conjugation of hydrophobic molecules to chitosan increased surface roughness and hydrophilicity, since it triggered a core-shell macromolecular structuration. Nanoindentation revealed a notable durotaxis gradient due to chitosan/fatty acid self-organization and graphene sheet embedment. The composited building blocks wiInternational journal of molecular sciencesChitosan-Based Materials Featuring Multiscale Anisotropy for Wider Tissue Engineering Applications.PMC9140409154/2016Popescu CCIonescu IBoerasu IGiol EDDumitrascu AMVlasceanu GMIonita MFloarea MOlaret EIovu HGhitman JNecolau MIfalseChitosan-Based Materials Featuring Multiscale Anisotropy for Wider Tissue Engineering Applications.We designed graphene oxide composites with increased morphological and structural variability using fatty acid-coupled polysaccharide co-polymer as the continuous phase. The matrix was synthesized by N, O-acylation of chitosan with palmitic and lauric acid. The obtained co-polymer was crosslinked with genipin and composited with graphene oxide. FTIR spectra highlighted the modification and multi-components interaction. DLS, SEM, and contact angle tests demonstrated that the conjugation of hydrophobic molecules to chitosan increased surface roughness and hydrophilicity, since it triggered a core-shell macromolecular structuration. Nanoindentation revealed a notable durotaxis gradient due to chitosan/fatty acid self-organization and graphene sheet embedment. The composited building blocks wi2022-01-01T00:00:00Z2022 May2025-05-29T14:36:29.45Z2025-02-19T02:16:24.131ZS-EPMC91404093562815010.3390/ijms23105336