biostudies-literatureAteeq MKing Saud University, Riyadh, Saudi Arabia3251https://www.ebi.ac.uk/biostudies/studies/S-EPMC9145308biostudies-literatureUnknown27(10)Vicilin has nutraceutical potential and different noteworthy medicative health-promoting biotic diversions, and it is remarkable against pathogenic microorganisms and insects. In this study, <i>Vigna aconitifolia</i> vicilin (<i>Vac</i>V) has been identified and characterized from the seed of <i>Vigna aconitifolia</i> (Jacq.) Marechal (Moth beans). LC-MS/MS analysis of <i>Vac</i>V provided seven random fragmented sequences comprising 238 residues, showing significant homology with already reported <i>Vigna radiata</i> vicilin (<i>Vra</i>V). <i>Vac</i>V was purified using ammonium sulfate precipitation (60%) followed by size exclusion chromatography on Hi-Load 16/60 Superdex 200 pg column and anion-exchange chromatography (Hi trap Q FF column). Purified <i>Vac</i>V showed a major ~50 kDa baMolecules (Basel, Switzerland)In Silico Analysis and Functional Characterization of Antimicrobial and Insecticidal Vicilin from Moth Bean (<i>Vigna aconitifolia</i> (Jacq.) Marechal) Seeds.PMC9145308RSP2022R491Akrem AAdeel MMTahir Ul Qamar MAlharbi MAtiq MNBilal MKhaliq BAteeq MSaeed ASaeed QKanwal AAlshammari AfalseIn Silico Analysis and Functional Characterization of Antimicrobial and Insecticidal Vicilin from Moth Bean (<i>Vigna aconitifolia</i> (Jacq.) Marechal) Seeds.Vicilin has nutraceutical potential and different noteworthy medicative health-promoting biotic diversions, and it is remarkable against pathogenic microorganisms and insects. In this study, <i>Vigna aconitifolia</i> vicilin (<i>Vac</i>V) has been identified and characterized from the seed of <i>Vigna aconitifolia</i> (Jacq.) Marechal (Moth beans). LC-MS/MS analysis of <i>Vac</i>V provided seven random fragmented sequences comprising 238 residues, showing significant homology with already reported <i>Vigna radiata</i> vicilin (<i>Vra</i>V). <i>Vac</i>V was purified using ammonium sulfate precipitation (60%) followed by size exclusion chromatography on Hi-Load 16/60 Superdex 200 pg column and anion-exchange chromatography (Hi trap Q FF column). Purified <i>Vac</i>V showed a major ~50 kDa ba2022-01-01T00:00:00Z2022 May2026-05-07T03:17:56.487Z2025-02-19T02:58:47.225ZS-EPMC91453083563072710.3390/molecules27103251