{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao X"],"funding":["Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["689"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9146380"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(5)"],"pubmed_abstract":["Thaxtomin A is a potent bioherbicide in both organic and conventional agriculture; however, its low yield hinders its wide application. Here, we report the direct cloning and heterologous expression of the thaxtomin A gene cluster in three well-characterized <i>Streptomyces</i> hosts. Then, we present an efficient, markerless and multiplex large gene cluster editing method based on in vitro CRISPR/Cas9 digestion and yeast homologous recombination. With this method, we successfully engineered the thaxtomin A cluster by simultaneously replacing the native promoters of the <i>txtED</i> operon, <i>txtABH</i> operon and <i>txtC</i> gene with strong constitutive promoters, and the yield of thaxtomin A improved to 289.5 µg/mL in heterologous <i>Streptomyces coelicolor</i> M1154. To further optimi"],"journal":["Life (Basel, Switzerland)"],"pubmed_title":["Multiplexed Promoter Engineering for Improving Thaxtomin A Production in Heterologous <i>Streptomyces</i> Hosts."],"pmcid":["PMC9146380"],"funding_grant_id":["32071412, 12090053, 32088101, 31500069","2018YFA0900700, 2020YFA0907101, 2021YFF1200500, 2015CB910300"],"pubmed_authors":["Zong Y","Lou C","Wei W","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"Multiplexed Promoter Engineering for Improving Thaxtomin A Production in Heterologous <i>Streptomyces</i> Hosts.","description":"Thaxtomin A is a potent bioherbicide in both organic and conventional agriculture; however, its low yield hinders its wide application. Here, we report the direct cloning and heterologous expression of the thaxtomin A gene cluster in three well-characterized <i>Streptomyces</i> hosts. Then, we present an efficient, markerless and multiplex large gene cluster editing method based on in vitro CRISPR/Cas9 digestion and yeast homologous recombination. With this method, we successfully engineered the thaxtomin A cluster by simultaneously replacing the native promoters of the <i>txtED</i> operon, <i>txtABH</i> operon and <i>txtC</i> gene with strong constitutive promoters, and the yield of thaxtomin A improved to 289.5 µg/mL in heterologous <i>Streptomyces coelicolor</i> M1154. To further optimi","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-22T18:22:06.163Z","creation":"2025-04-06T02:23:02.01Z"},"accession":"S-EPMC9146380","cross_references":{"pubmed":["35629358"],"doi":["10.3390/life12050689"]}}