biostudies-literatureMeng JFund for Distinguish Young Scholars in Tianjin 2018 3rd RoundHebei Science and Technology FoundationHebei Natural Science FoundationJiangsu Key Laboratory of Advanced Manufacturing Equipment and TechnologyNational Natural Science Foundation of ChinaDepartment of Human Resources and Social Security of Hebei Province792https://www.ebi.ac.uk/biostudies/studies/S-EPMC9146403biostudies-literatureUnknown13(5)Microfluidic devices offer excellent heat transfer, enabling the biochemical reactions to be more efficient. However, the precision of temperature sensing and control of microfluids is limited by the size effect. Here in this work, the relationship between the microfluids and the glass substrate of a typical microfluidic device is investigated. With an intelligent structure design and liquid metal, we demonstrated that a millimeter-scale industrial temperature sensor could be utilized for temperature sensing of micro-scale fluids. We proposed a heat transfer model based on this design, where the local correlations between the macro-scale temperature sensor and the micro-scale fluids were investigated. As a demonstration, a set of temperature-sensitive nucleic acid amplification tests were MicromachinesMicrofluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer.PMC914640319271707D51728502C20200314 and C20210337180191FMZ202016E2020202101, E2022202127 and F2021202001Li HLi JLi SDai SYu CWei CMeng JfalseMicrofluidics Temperature Compensating and Monitoring Based on Liquid Metal Heat Transfer.Microfluidic devices offer excellent heat transfer, enabling the biochemical reactions to be more efficient. However, the precision of temperature sensing and control of microfluids is limited by the size effect. Here in this work, the relationship between the microfluids and the glass substrate of a typical microfluidic device is investigated. With an intelligent structure design and liquid metal, we demonstrated that a millimeter-scale industrial temperature sensor could be utilized for temperature sensing of micro-scale fluids. We proposed a heat transfer model based on this design, where the local correlations between the macro-scale temperature sensor and the micro-scale fluids were investigated. As a demonstration, a set of temperature-sensitive nucleic acid amplification tests were 2022-01-01T00:00:00Z2022 May2025-04-26T02:40:12.12Z2025-02-19T02:15:58.171ZS-EPMC91464033563025910.3390/mi13050792