biostudies-literatureWang Cthe Modern Dental Laboratory/HKU Endowment Fund to L.J. Jinthe Hong Kong Research Grants Council5728https://www.ebi.ac.uk/biostudies/studies/S-EPMC9146424biostudies-literatureUnknown23(10)<i>Porphyromonas gingivalis</i> as the keystone periodontopathogen plays a critical role in the pathogenesis of periodontitis, and crucially accounts for inflammatory comorbidities such as cardiovascular disease and Alzheimer's disease. We recently identified the existence of <i>P. gingivalis</i> persisters and revealed the unforeseen perturbation of innate response in human gingival epithelial cells (HGECs) due to these noxious persisters. Herein, RNA sequencing revealed how <i>P. gingivalis</i> persisters affected the expression profile of cytokine genes and related signaling pathways in HGECs. Results showed that metronidazole-treated <i>P. gingivalis</i> persisters (M-PgPs) impaired the innate host defense of HGECs, in a similar fashion to <i>P. gingivalis</i>. Notably, over one thousaInternational journal of molecular sciencesRNA Sequencing Reveals the Upregulation of FOXO Signaling Pathway in <i>Porphyromonas gingivalis</i> Persister-Treated Human Gingival Epithelial Cells.PMC9146424Nil17122918, 17119819Li XCheng TWang CWang LJin LfalseRNA Sequencing Reveals the Upregulation of FOXO Signaling Pathway in <i>Porphyromonas gingivalis</i> Persister-Treated Human Gingival Epithelial Cells.<i>Porphyromonas gingivalis</i> as the keystone periodontopathogen plays a critical role in the pathogenesis of periodontitis, and crucially accounts for inflammatory comorbidities such as cardiovascular disease and Alzheimer's disease. We recently identified the existence of <i>P. gingivalis</i> persisters and revealed the unforeseen perturbation of innate response in human gingival epithelial cells (HGECs) due to these noxious persisters. Herein, RNA sequencing revealed how <i>P. gingivalis</i> persisters affected the expression profile of cytokine genes and related signaling pathways in HGECs. Results showed that metronidazole-treated <i>P. gingivalis</i> persisters (M-PgPs) impaired the innate host defense of HGECs, in a similar fashion to <i>P. gingivalis</i>. Notably, over one thousa2022-01-01T00:00:00Z2022 May2025-05-29T14:36:24.83Z2025-02-19T02:16:20.636ZS-EPMC91464243562854210.3390/ijms23105728