biostudies-literatureUnknown10(5)Attar RDue to the misuse of antibiotics in our daily lives, antimicrobial resistance (AMR) has become a major health problem. Penicillin, the first antibiotic, was used in the 1930s and led to the emergence of AMR. Due to alterations in the microbe's genome and the evolution of new resistance mechanisms, antibiotics are losing efficacy against microbes. There are high rates of mortality and morbidity due to antibiotic resistance, so addressing this major health issue requires new approaches. <i>Staphylococcus auricularis</i> is a Gram-positive cocci and is capable of causing opportunistic infections and sepsis. <i>S. auricularis</i> is resistant to several antibiotics and does not currently have a licensed vaccine. In this study, we used bacterial pan-genome analysis (BPGA) to study <i>S. auriculVaccines637https://www.ebi.ac.uk/biostudies/studies/S-EPMC9146471biostudies-literatureImmunoinformatics and Biophysics Approaches to Design a Novel Multi-Epitopes Vaccine Design against <i>Staphylococcus auricularis</i>.PMC9146471Attar RAlharbi KNAlatawi EAAlmatroudi AAba Alkhayl FFAllemailem KSfalseImmunoinformatics and Biophysics Approaches to Design a Novel Multi-Epitopes Vaccine Design against <i>Staphylococcus auricularis</i>.Due to the misuse of antibiotics in our daily lives, antimicrobial resistance (AMR) has become a major health problem. Penicillin, the first antibiotic, was used in the 1930s and led to the emergence of AMR. Due to alterations in the microbe's genome and the evolution of new resistance mechanisms, antibiotics are losing efficacy against microbes. There are high rates of mortality and morbidity due to antibiotic resistance, so addressing this major health issue requires new approaches. <i>Staphylococcus auricularis</i> is a Gram-positive cocci and is capable of causing opportunistic infections and sepsis. <i>S. auricularis</i> is resistant to several antibiotics and does not currently have a licensed vaccine. In this study, we used bacterial pan-genome analysis (BPGA) to study <i>S. auricul2022-01-01T00:00:00Z2022 Apr2025-04-26T13:02:22.243Z2025-04-06T14:08:44.962ZS-EPMC91464713563239410.3390/vaccines10050637