biostudies-literatureLv ZNational Key Research and Development Program of China971https://www.ebi.ac.uk/biostudies/studies/S-EPMC9146948biostudies-literatureUnknown10(5)This study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against <i>Botryris cinerea</i>. The combined bio-fungicide achieved an inhibition rate of 100% for mycelial growth using ε-PL at 500 μg/mL + wuyiencin at 50 μg/mL and for spore germination using ε-PL at 200 μg/mL + wuyiencin at 80 μg/mL in vitro. This synergistic spore and mycelia-damaging effect of the combination was confirmed using scanning electron microscopy. In vivo assays with combined bio-fungicide (1500 μg/mL ε-PL + 60 μg/mL wuyiencin) on detached leaves showed depressed growth and development of the spores of <i>B. cinerea</i>. The synergistic effect was further tested in MicroorganismsEffects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i>.PMC9146948No. 2019YFD1002000Lv ZGe BShi LZhang KLu YLi BfalseEffects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i>.This study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against <i>Botryris cinerea</i>. The combined bio-fungicide achieved an inhibition rate of 100% for mycelial growth using ε-PL at 500 μg/mL + wuyiencin at 50 μg/mL and for spore germination using ε-PL at 200 μg/mL + wuyiencin at 80 μg/mL in vitro. This synergistic spore and mycelia-damaging effect of the combination was confirmed using scanning electron microscopy. In vivo assays with combined bio-fungicide (1500 μg/mL ε-PL + 60 μg/mL wuyiencin) on detached leaves showed depressed growth and development of the spores of <i>B. cinerea</i>. The synergistic effect was further tested in 2022-01-01T00:00:00Z2022 May2025-04-22T18:23:11.248Z2025-04-06T02:24:32.945ZS-EPMC91469483563041810.3390/microorganisms10050971