{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ribani A"],"funding":["Regione Emilia-Romagna","University of Bologna"],"pagination":["213"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9147136"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(5)"],"pubmed_abstract":["Environmental DNA (eDNA) contained in honey derives from the organisms that directly and indirectly have been involved in the production process of this matrix and that have played a role in the hive ecosystems where the honey has been produced. In this study we set up PCR-based assays to detect the presence of DNA traces left in the honey by two damaging honey bee pests: the small hive beetle (<i>Aethina tumida</i>) and the greater wax moth (<i>Galleria mellonella</i>). DNA was extracted from 82 honey samples produced in Italy and amplified using two specific primer pairs that target the mitochondrial gene cytochrome oxidase I (COI) of <i>A. tumida</i> and two specific primer pairs that target the same gene in <i>G. mellonella</i>. The limit of detection was tested using sequential diluti"],"journal":["Veterinary sciences"],"pubmed_title":["Honey Environmental DNA Can Be Used to Detect and Monitor Honey Bee Pests: Development of Methods Useful to Identify <i>Aethina tumida</i> and <i>Galleria mellonella</i> Infestations."],"pmcid":["PMC9147136"],"funding_grant_id":["BEE-RER-3 project- CUP E37G22000030007-del Regolamento (UE) no. 1308/2013 -(OCM Apicoltura)","RFO 2020-2021"],"pubmed_authors":["Taurisano V","Ribani A","Utzeri VJ","Fontanesi L"],"additional_accession":[]},"is_claimable":false,"name":"Honey Environmental DNA Can Be Used to Detect and Monitor Honey Bee Pests: Development of Methods Useful to Identify <i>Aethina tumida</i> and <i>Galleria mellonella</i> Infestations.","description":"Environmental DNA (eDNA) contained in honey derives from the organisms that directly and indirectly have been involved in the production process of this matrix and that have played a role in the hive ecosystems where the honey has been produced. In this study we set up PCR-based assays to detect the presence of DNA traces left in the honey by two damaging honey bee pests: the small hive beetle (<i>Aethina tumida</i>) and the greater wax moth (<i>Galleria mellonella</i>). DNA was extracted from 82 honey samples produced in Italy and amplified using two specific primer pairs that target the mitochondrial gene cytochrome oxidase I (COI) of <i>A. tumida</i> and two specific primer pairs that target the same gene in <i>G. mellonella</i>. The limit of detection was tested using sequential diluti","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2026-05-06T03:20:50.017Z","creation":"2025-05-18T12:05:09.276Z"},"accession":"S-EPMC9147136","cross_references":{"pubmed":["35622741"],"doi":["10.3390/vetsci9050213"]}}