biostudies-literatureRibeiro DLPublic Ministry of Labor Campinas (Research, Prevention and Education of Occupational Cancer)São Paulo Research FoundationFAPESPState University of Londrina (UEL)Coordination for the Improvement of Higher Education PersonnelCoordenação de Aperfeicoamento de Pessoal de Nível SuperiorUniversity of São Paulo (USP)Maranhão Foundation for Research and Scientific and Technological DevelopmentNational Council for Scientific and Technological DevelopmentFundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão963https://www.ebi.ac.uk/biostudies/studies/S-EPMC9147598biostudies-literatureUnknown14(5)Metastatic prostate cancer (mPCa) is resistant to several chemotherapeutic agents. Brachydin A (BrA), a glycosylated flavonoid extracted from <i>Fridericia platyphylla</i>, displays a remarkable antitumoral effect against in vitro mPCa cells cultured as bidimensional (2D) monolayers. Considering that three-dimensional (3D) cell cultures provide a more accurate response to chemotherapeutic agents, this study investigated the antiproliferative/antimetastatic effects of BrA and the molecular mechanisms underlying its action in mPCa spheroids (DU145) in vitro. BrA at 60-100 μM was cytotoxic, altered spheroid morphology/volume, and suppressed cell migration and tumor invasiveness. High-content analysis revealed that BrA (60-100 µM) reduced mitochondrial membrane potential and increased apoptosiPharmaceuticsThe Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death.PMC9147598# 00849/18Research Productivity grant # 308231/2017# 2017/22305-9National Postdoctoral Program PNPD grant # 88887.354023/2019 and Finance Code 001Postdoctoral Fellowship grant #151520/2018-6 and Universal Project grant # 426246/2018-7grant # 00849/18Postdoctoral Fellowship grant #151520/2018-6Universal Project grant # 426246/2018-7Rocha CQDAntunes LMGTuttis KOliveira LCBGomes INFLengert AVHSerpeloni JMRibeiro DLReis RMColus IMSfalseThe Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death.Metastatic prostate cancer (mPCa) is resistant to several chemotherapeutic agents. Brachydin A (BrA), a glycosylated flavonoid extracted from <i>Fridericia platyphylla</i>, displays a remarkable antitumoral effect against in vitro mPCa cells cultured as bidimensional (2D) monolayers. Considering that three-dimensional (3D) cell cultures provide a more accurate response to chemotherapeutic agents, this study investigated the antiproliferative/antimetastatic effects of BrA and the molecular mechanisms underlying its action in mPCa spheroids (DU145) in vitro. BrA at 60-100 μM was cytotoxic, altered spheroid morphology/volume, and suppressed cell migration and tumor invasiveness. High-content analysis revealed that BrA (60-100 µM) reduced mitochondrial membrane potential and increased apoptosi2022-01-01T00:00:00Z2022 Apr2026-06-21T03:16:37.653Z2024-11-05T20:39:15.775ZS-EPMC91475983563155010.3390/pharmaceutics14050963