{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(1)"],"submitter":["Lin S"],"pubmed_abstract":["Background
The transforming growth factor-beta (TGF-β) signaling pathway is an important pathway associated with the pathogenesis of osteoarthritis (OA). This study was to investigate the involvement of circRNAs in the TGF-β signaling pathway.Methods
Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) assay were used to detect the proliferation of primary mouse chondrocytes (PMCs). RNA-sequencing together with bioinformatics analysis were used to systematically clarify TGF-β1 induced alternations of circRNAs in PMCs. The regulatory and functional role of circPhf21a was examined in PMCs. Downstream targets of circPhf21a were explored by RNA-sequencing after overexpression of circPhf21a and verified by RT-qPCR in PMCs. Finally, the role and mechanism of circ"],"journal":["Cell communication and signaling : CCS"],"pagination":["75"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9150374"],"repository":["biostudies-literature"],"pubmed_title":["TGF-β1 regulates chondrocyte proliferation and extracellular matrix synthesis via circPhf21a-Vegfa axis in osteoarthritis."],"pmcid":["PMC9150374"],"pubmed_authors":["Li H","Lin S","Lu H","Peng R","Shang J","Wu B","Xing B","Xu X","Jiang N"],"additional_accession":[]},"is_claimable":false,"name":"TGF-β1 regulates chondrocyte proliferation and extracellular matrix synthesis via circPhf21a-Vegfa axis in osteoarthritis.","description":"Background
The transforming growth factor-beta (TGF-β) signaling pathway is an important pathway associated with the pathogenesis of osteoarthritis (OA). This study was to investigate the involvement of circRNAs in the TGF-β signaling pathway.Methods
Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) assay were used to detect the proliferation of primary mouse chondrocytes (PMCs). RNA-sequencing together with bioinformatics analysis were used to systematically clarify TGF-β1 induced alternations of circRNAs in PMCs. The regulatory and functional role of circPhf21a was examined in PMCs. Downstream targets of circPhf21a were explored by RNA-sequencing after overexpression of circPhf21a and verified by RT-qPCR in PMCs. Finally, the role and mechanism of circ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 May","modification":"2025-04-04T09:41:34.754Z","creation":"2025-04-04T09:41:34.754Z"},"accession":"S-EPMC9150374","cross_references":{"pubmed":["35637489"],"doi":["10.1186/s12964-022-00881-9"]}}