{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Viegas IJ"],"funding":["NINDS NIH HHS","NCI NIH HHS","Engineering and Physical Sciences Research Council"],"pagination":["362-370"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9150445"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["604(7905)"],"pubmed_abstract":["RNA modifications are important regulators of gene expression<sup>1</sup>. In Trypanosoma brucei, transcription is polycistronic and thus most regulation happens post-transcriptionally<sup>2</sup>. N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) has been detected in this parasite, but its function remains unknown<sup>3</sup>. Here we found that m<sup>6</sup>A is enriched in 342 transcripts using RNA immunoprecipitation, with an enrichment in transcripts encoding variant surface glycoproteins (VSGs). Approximately 50% of the m<sup>6</sup>A is located in the poly(A) tail of the actively expressed VSG transcripts. m<sup>6</sup>A residues are removed from the VSG poly(A) tail before deadenylation and mRNA degradation. Computational analysis revealed an association between m<sup>6</sup>A in the "],"journal":["Nature"],"pubmed_title":["N<sup>6</sup>-methyladenosine in poly(A) tails stabilize VSG transcripts."],"pmcid":["PMC9150445"],"funding_grant_id":["T32 CA062948","R35 NS111631","EP/K039660/1","EP/M028127/1"],"pubmed_authors":["De Niz M","Serra L","Silva Pereira S","Viegas IJ","Temporao A","Bergstrom E","Figueiredo LM","Aresta-Branco F","Jaffrey SR","de Macedo JP","Mirza AH","Rodrigues JA"],"additional_accession":[]},"is_claimable":false,"name":"N<sup>6</sup>-methyladenosine in poly(A) tails stabilize VSG transcripts.","description":"RNA modifications are important regulators of gene expression<sup>1</sup>. In Trypanosoma brucei, transcription is polycistronic and thus most regulation happens post-transcriptionally<sup>2</sup>. N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) has been detected in this parasite, but its function remains unknown<sup>3</sup>. Here we found that m<sup>6</sup>A is enriched in 342 transcripts using RNA immunoprecipitation, with an enrichment in transcripts encoding variant surface glycoproteins (VSGs). Approximately 50% of the m<sup>6</sup>A is located in the poly(A) tail of the actively expressed VSG transcripts. m<sup>6</sup>A residues are removed from the VSG poly(A) tail before deadenylation and mRNA degradation. Computational analysis revealed an association between m<sup>6</sup>A in the ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-04T12:05:11.193Z","creation":"2025-04-04T12:05:11.193Z"},"accession":"S-EPMC9150445","cross_references":{"pubmed":["35355019"],"doi":["10.1038/s41586-022-04544-0"]}}