biostudies-literatureXu MNCI NIH HHS45-52https://www.ebi.ac.uk/biostudies/studies/S-EPMC9153261biostudies-literatureUnknown4(2)<h4>Introduction</h4>For maximum utility of molecular characterization by next-generation sequencing (NGS) and better understanding of tumor microenvironment with immune correlates analysis, biopsy specimens must yield adequate tumor tissue, and sequential biopsy specimens should sample a consistent site. We developed a web-based lesion selection tool (LST) that enables management and tracking of the biopsy specimen collections.<h4>Methods</h4>Of 145 patients, the LST was used for 88 patients; the other 57 served as controls. We evaluated consistency of the lesion biopsied in longitudinal collections, number of cores obtained, and cores with adequate tumor cellularity for NGS. The Fisher exact test and Wilcoxon rank sum test were used to identify differences between the groups.<h4>Results<Journal of immunotherapy and precision oncologyImplementation of a Novel Web-Based Lesion Selection Tool to Improve Acquisition of Tumor Biopsy Specimens.PMC9153261P30 CA016672Hajjar JFu SPant SNagarajan PCarter BRoy-Chowdhuri SSabir STapia CStephen BSun RGong JKarp DDJavle MKoay EJXu MMeric-Bernstam FLe HWistuba IIAung PPRodon JYang YAbdelsalam MEJhingran AColen RNaing AHwu PMilton DRZarifa AYang VfalseImplementation of a Novel Web-Based Lesion Selection Tool to Improve Acquisition of Tumor Biopsy Specimens.<h4>Introduction</h4>For maximum utility of molecular characterization by next-generation sequencing (NGS) and better understanding of tumor microenvironment with immune correlates analysis, biopsy specimens must yield adequate tumor tissue, and sequential biopsy specimens should sample a consistent site. We developed a web-based lesion selection tool (LST) that enables management and tracking of the biopsy specimen collections.<h4>Methods</h4>Of 145 patients, the LST was used for 88 patients; the other 57 served as controls. We evaluated consistency of the lesion biopsied in longitudinal collections, number of cores obtained, and cores with adequate tumor cellularity for NGS. The Fisher exact test and Wilcoxon rank sum test were used to identify differences between the groups.<h4>Results<2021-01-01T00:00:00Z2021 May2026-05-27T20:31:44.06Z2025-02-19T02:44:23.203ZS-EPMC91532613566353110.36401/JIPO-21-510.36401/jipo-21-5