biostudies-literatureMacPherson DSNIBIB NIH HHSAir Force Research LaboratoryNCI NIH HHSNational Science Foundation579-587https://www.ebi.ac.uk/biostudies/studies/S-EPMC9153392biostudies-literatureUnknown8(2)Matrix metalloproteinase (MMP) enzymes are over-expressed by some metastatic cancers, in which they are responsible for the degradation and remodeling of the extracellular matrix. In recent years, MMPs have emerged as promising targets for enzyme-responsive diagnostic probes because oligopeptides can be designed to be selectively hydrolyzed by exposure to these enzymes. With the ultimate goal of developing radio-iodinated peptides as supramolecular building blocks for MMP-sensitive tools for nuclear imaging and therapy, we designed three MMP-9-responsive peptides containing either tyrosine or iodotyrosine to assess the impact of iodotyrosine introduction to the peptide structure and cleavage kinetics. We found that the peptides containing iodotyrosine underwent more rapid and more completeACS biomaterials science & engineeringThe Impact of Tyrosine Iodination on the Aggregation and Cleavage Kinetics of MMP-9-Responsive Peptide Sequences.PMC9153392R01 CA204167P30 CA008748R21 EB030275FA9550-19-1-0111HRD-1547830R01 CA240963U01 CA221046McPhee SAMacPherson DSUlijn RVZeglis BMfalseThe Impact of Tyrosine Iodination on the Aggregation and Cleavage Kinetics of MMP-9-Responsive Peptide Sequences.Matrix metalloproteinase (MMP) enzymes are over-expressed by some metastatic cancers, in which they are responsible for the degradation and remodeling of the extracellular matrix. In recent years, MMPs have emerged as promising targets for enzyme-responsive diagnostic probes because oligopeptides can be designed to be selectively hydrolyzed by exposure to these enzymes. With the ultimate goal of developing radio-iodinated peptides as supramolecular building blocks for MMP-sensitive tools for nuclear imaging and therapy, we designed three MMP-9-responsive peptides containing either tyrosine or iodotyrosine to assess the impact of iodotyrosine introduction to the peptide structure and cleavage kinetics. We found that the peptides containing iodotyrosine underwent more rapid and more complete2022-01-01T00:00:00Z2022 Feb2025-05-18T12:48:15.794Z2025-04-04T19:47:38.454ZS-EPMC91533923505057410.1021/acsbiomaterials.1c01488